Mapping a double flower phenotype-associated gene DcAP2L in Dianthus chinensis.
APETALA2 (DcAP2L)
Dianthus chinensis
BSR-seq
QTL mapping
ddRAD
double flower trait
miR172
Journal
Journal of experimental botany
ISSN: 1460-2431
Titre abrégé: J Exp Bot
Pays: England
ID NLM: 9882906
Informations de publication
Date de publication:
25 03 2020
25 03 2020
Historique:
received:
23
05
2019
accepted:
25
01
2020
pubmed:
29
1
2020
medline:
15
5
2021
entrez:
29
1
2020
Statut:
ppublish
Résumé
The double flower is a highly important breeding trait that affects the ornamental value in many flowering plants. To get a better understanding of the genetic mechanism of double flower formation in Dianthus chinensis, we have constructed a high-density genetic map using 140 F2 progenies derived from a cross between a single flower genotype and a double flower genotype. The linkage map was constructed using double-digest restriction site-associated DNA sequencing (ddRAD-seq) with 2353 single nucleotide polymorphisms (SNPs). Quantitative trait locus (QTL) mapping analysis was conducted for 12 horticultural traits, and major QTLs were identified for nine of the 12 traits. Among them, two major QTLs accounted for 20.7% and 78.1% of the total petal number variation, respectively. Bulked segregant RNA-seq (BSR-seq) was performed to search accurately for candidate genes associated with the double flower trait. Integrative analysis of QTL mapping and BSR-seq analysis using the reference genome of Dianthus caryophyllus suggested that an SNP mutation in the miR172 cleavage site of the A-class flower organ identity gene APETALA2 (DcAP2L) is responsible for double flower formation in Dianthus through regulating the expression of DcAG genes.
Identifiants
pubmed: 31990971
pii: 5716848
doi: 10.1093/jxb/erz558
pmc: PMC7242084
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1915-1927Informations de copyright
© The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissions@oup.com.
Références
PLoS One. 2012;7(5):e37135
pubmed: 22675423
Plant Physiol. 2006 Feb;140(2):411-32
pubmed: 16407444
Nat Methods. 2012 May 20;9(8):808-10
pubmed: 22609625
Plant Sci. 2014 Feb;215-216:29-38
pubmed: 24388512
PLoS One. 2009;4(5):e5553
pubmed: 19436741
Plant Cell. 1989 Jan;1(1):37-52
pubmed: 2535466
PLoS Genet. 2013;9(1):e1003218
pubmed: 23349639
Curr Opin Genet Dev. 2005 Aug;15(4):454-60
pubmed: 15964755
Plant J. 2018 Oct;96(2):358-371
pubmed: 30047177
Science. 2004 Mar 26;303(5666):2022-5
pubmed: 12893888
PLoS One. 2015 Sep 23;10(9):e0137746
pubmed: 26398886
Genomics. 1997 Nov 15;46(1):24-36
pubmed: 9403055
Evodevo. 2010 Dec 01;1:13
pubmed: 21122096
BMC Plant Biol. 2012 Aug 21;12:149
pubmed: 22909020
Development. 2017 Jun 1;144(11):1959-1965
pubmed: 28455374
Development. 2010 Nov;137(21):3633-42
pubmed: 20876650
Front Plant Sci. 2018 Mar 27;9:398
pubmed: 29636767
Genome Res. 2010 Sep;20(9):1297-303
pubmed: 20644199
Bioinformatics. 2014 Aug 1;30(15):2114-20
pubmed: 24695404
PLoS One. 2016 Jan 05;11(1):e0146383
pubmed: 26730738
BMC Genomics. 2014 Mar 26;15:233
pubmed: 24669946
J Exp Bot. 2013 Feb;64(4):847-57
pubmed: 23364936
Mol Phylogenet Evol. 2016 Jan;94(Pt A):122-35
pubmed: 26279345
Mol Ecol. 2013 Jun;22(11):3124-40
pubmed: 23701397
Proc Natl Acad Sci U S A. 2012 Aug 21;109(34):E2267-75
pubmed: 22853954
New Phytol. 2017 Aug;215(3):1197-1209
pubmed: 27604611
Genetics. 1986 Apr;112(4):887-98
pubmed: 17246322
Mol Biol Evol. 2013 Dec;30(12):2725-9
pubmed: 24132122
Am J Bot. 2003 Mar;90(3):461-70
pubmed: 21659139
Gene. 2006 Feb 1;366(2):256-65
pubmed: 16388920
ACS Synth Biol. 2014 Nov 21;3(11):839-43
pubmed: 24933124
Genes (Basel). 2018 Apr 04;9(4):
pubmed: 29617274
Development. 1991 May;112(1):1-20
pubmed: 1685111
Mol Ecol. 2016 May;25(10):2165-75
pubmed: 26923740
Planta. 2007 Feb;225(3):589-602
pubmed: 16953432
Sci Rep. 2013;3:2641
pubmed: 24026510
Cell. 1991 Jun 14;65(6):991-1002
pubmed: 1675158
Development. 2012 Nov;139(22):4180-90
pubmed: 23034631
Brief Funct Genomics. 2010 Dec;9(5-6):416-23
pubmed: 21266344
PLoS One. 2013 Dec 06;8(12):e82333
pubmed: 24324772
PLoS One. 2010 Feb 18;5(2):e9288
pubmed: 20174587
Mol Biol Evol. 2006 Jan;23(1):107-20
pubmed: 16151182
PLoS One. 2012;7(5):e36406
pubmed: 22586469
Nat Rev Genet. 2005 Sep;6(9):688-98
pubmed: 16151374
Nat Protoc. 2006;1(4):2019-25
pubmed: 17487191
Plant J. 2007 Sep;51(5):840-9
pubmed: 17573799
Sci Rep. 2018 Aug 27;8(1):12912
pubmed: 30150746
Development. 2017 Jun 1;144(11):1966-1975
pubmed: 28455375
BMC Mol Biol. 2010 Jan 13;11:1
pubmed: 20070894