Characterization and diversity of the complete set of GH family 3 enzymes from Rhodothermus marinus DSM 4253.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
28 Jan 2020
28 Jan 2020
Historique:
received:
19
09
2019
accepted:
07
01
2020
entrez:
30
1
2020
pubmed:
30
1
2020
medline:
11
6
2020
Statut:
epublish
Résumé
The genome of Rhodothermus marinus DSM 4253 encodes six glycoside hydrolases (GH) classified under GH family 3 (GH3): RmBgl3A, RmBgl3B, RmBgl3C, RmXyl3A, RmXyl3B and RmNag3. The biochemical function, modelled 3D-structure, gene cluster and evolutionary relationships of each of these enzymes were studied. The six enzymes were clustered into three major evolutionary lineages of GH3: β-N-acetyl-glucosaminidases, β-1,4-glucosidases/β-xylosidases and macrolide β-glucosidases. The RmNag3 with additional β-lactamase domain clustered with the deepest rooted GH3-lineage of β-N-acetyl-glucosaminidases and was active on acetyl-chitooligosaccharides. RmBgl3B displayed β-1,4-glucosidase activity and was the only representative of the lineage clustered with macrolide β-glucosidases from Actinomycetes. The β-xylosidases, RmXyl3A and RmXyl3B, and the β-glucosidases RmBgl3A and RmBgl3C clustered within the major β-glucosidases/β-xylosidases evolutionary lineage. RmXyl3A and RmXyl3B showed β-xylosidase activity with different specificities for para-nitrophenyl (pNP)-linked substrates and xylooligosaccharides. RmBgl3A displayed β-1,4-glucosidase/β-xylosidase activity while RmBgl3C was active on pNP-β-Glc and β-1,3-1,4-linked glucosyl disaccharides. Putative polysaccharide utilization gene clusters were also investigated for both R. marinus DSM 4253 and DSM 4252
Identifiants
pubmed: 31992772
doi: 10.1038/s41598-020-58015-5
pii: 10.1038/s41598-020-58015-5
pmc: PMC6987092
doi:
Substances chimiques
Glycoside Hydrolases
EC 3.2.1.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1329Subventions
Organisme : EC | Horizon 2020 Framework Programme (EU Framework Programme for Research and Innovation H2020)
ID : 720755
Organisme : EC | Horizon 2020 Framework Programme (EU Framework Programme for Research and Innovation H2020)
ID : 727473
Organisme : EC | Horizon 2020 Framework Programme (EU Framework Programme for Research and Innovation H2020)
ID : 720755
Organisme : EC | Horizon 2020 Framework Programme (EU Framework Programme for Research and Innovation H2020)
ID : 727473
Organisme : EC | EC Seventh Framework Programm | FP7 Food, Agriculture and Fisheries, Biotechnology (FP7-KBBE - Specific Programme "Cooperation": Food, Agriculture and Fisheries, Biotechnology)
ID : 604814
Organisme : EC | EC Seventh Framework Programm | FP7 Food, Agriculture and Fisheries, Biotechnology (FP7-KBBE - Specific Programme "Cooperation": Food, Agriculture and Fisheries, Biotechnology)
ID : 604814
Organisme : Svenska Forskningsrådet Formas (Swedish Research Council Formas)
ID : 2015-769
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