uPAR Knockout Results in a Deep Glycolytic and OXPHOS Reprogramming in Melanoma and Colon Carcinoma Cell Lines.
Base Sequence
CRISPR-Associated Protein 9
/ metabolism
Cell Line, Tumor
Cell Respiration
/ genetics
Colonic Neoplasms
/ genetics
Deoxyribonuclease I
/ metabolism
Fluorescence
Gene Expression Regulation, Neoplastic
Gene Knockout Techniques
Glycolysis
/ genetics
Humans
Lactic Acid
/ metabolism
Melanoma
/ genetics
Mitochondria
/ metabolism
Organelle Biogenesis
Oxidative Phosphorylation
RNA, Guide, Kinetoplastida
/ genetics
Receptors, Urokinase Plasminogen Activator
/ genetics
Stress, Physiological
CRISPR
colon cancer
gene-editing
melanoma
uPAR
Journal
Cells
ISSN: 2073-4409
Titre abrégé: Cells
Pays: Switzerland
ID NLM: 101600052
Informations de publication
Date de publication:
28 01 2020
28 01 2020
Historique:
received:
20
12
2019
revised:
24
01
2020
accepted:
26
01
2020
entrez:
5
2
2020
pubmed:
6
2
2020
medline:
13
2
2021
Statut:
epublish
Résumé
Urokinase Plasminogen Activator (uPA) Receptor (uPAR) is a well-known GPI-anchored three-domain membrane protein with pro-tumor roles largely shown in all the malignant tumors where it is over-expressed. Here we have exploited the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 gene knock out approach to investigate its role in the oxidative metabolism in human melanoma and colon cancer as the consequences of its irreversible loss. Knocking out
Identifiants
pubmed: 32012858
pii: cells9020308
doi: 10.3390/cells9020308
pmc: PMC7072355
pii:
doi:
Substances chimiques
RNA, Guide
0
Receptors, Urokinase Plasminogen Activator
0
Lactic Acid
33X04XA5AT
CRISPR-Associated Protein 9
EC 3.1.-
Deoxyribonuclease I
EC 3.1.21.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
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