DNA ADP-Ribosylation Stalls Replication and Is Reversed by RecF-Mediated Homologous Recombination and Nucleotide Excision Repair.
ADP-Ribosylation
Adenosine Diphosphate Ribose
/ metabolism
DNA Repair
DNA Replication
DNA, Bacterial
/ metabolism
DNA-Binding Proteins
/ metabolism
Enteropathogenic Escherichia coli
/ metabolism
Escherichia coli Proteins
/ metabolism
Homologous Recombination
Microbial Viability
Models, Biological
SOS Response, Genetics
DNA ADP-ribosylation
DNA damage
EPEC
SOS response
nucleotide excision repair
toxin-antitoxin system
Journal
Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691
Informations de publication
Date de publication:
04 02 2020
04 02 2020
Historique:
received:
24
07
2019
revised:
16
10
2019
accepted:
02
01
2020
entrez:
6
2
2020
pubmed:
6
2
2020
medline:
17
3
2021
Statut:
ppublish
Résumé
ADP-ribosylation of proteins is crucial for fundamental cellular processes. Despite increasing examples of DNA ADP-ribosylation, the impact of this modification on DNA metabolism and cell physiology is unknown. Here, we show that the DarTG toxin-antitoxin system from enteropathogenic Escherichia coli (EPEC) catalyzes reversible ADP-ribosylation of single-stranded DNA (ssDNA). The DarT toxin recognizes specific sequence motifs. EPEC DarG abrogates DarT toxicity by two distinct mechanisms: removal of DNA ADP-ribose (ADPr) groups and DarT sequestration. Furthermore, we investigate how cells recognize and deal with DNA ADP-ribosylation. We demonstrate that DNA ADPr stalls replication and is perceived as DNA damage. Removal of ADPr from DNA requires the sequential activity of two DNA repair pathways, with RecF-mediated homologous recombination likely to transfer ADP-ribosylation from single- to double-stranded DNA (dsDNA) and subsequent nucleotide excision repair eliminating the lesion. Our work demonstrates that these DNA repair pathways prevent the genotoxic effects of DNA ADP-ribosylation.
Identifiants
pubmed: 32023456
pii: S2211-1247(20)30023-1
doi: 10.1016/j.celrep.2020.01.014
pmc: PMC7003065
pii:
doi:
Substances chimiques
DNA, Bacterial
0
DNA-Binding Proteins
0
Escherichia coli Proteins
0
recF protein, E coli
0
Adenosine Diphosphate Ribose
20762-30-5
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1373-1384.e4Subventions
Organisme : Wellcome Trust
ID : 102908/Z/13/Z
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
Pays : United Kingdom
Informations de copyright
Copyright © 2020 The Author(s). Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Interests The authors declare no competing interests.
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