Muscarinic and Nicotinic Modulation of Neocortical Layer 6A Synaptic Microcircuits Is Cooperative and Cell-Specific.
Acetylcholine
/ metabolism
Animals
Cholinergic Agonists
/ pharmacology
Excitatory Postsynaptic Potentials
Glutamic Acid
/ metabolism
Neocortex
/ drug effects
Neural Pathways
Patch-Clamp Techniques
Pyramidal Cells
/ drug effects
Rats
Receptor, Muscarinic M1
/ drug effects
Receptor, Muscarinic M4
/ drug effects
Receptors, Muscarinic
/ drug effects
Receptors, Nicotinic
/ drug effects
Synaptic Transmission
/ drug effects
Thalamus
acetylcholine
barrel cortex
layer 6
muscarinic receptors
nicotinic receptors
Journal
Cerebral cortex (New York, N.Y. : 1991)
ISSN: 1460-2199
Titre abrégé: Cereb Cortex
Pays: United States
ID NLM: 9110718
Informations de publication
Date de publication:
18 05 2020
18 05 2020
Historique:
received:
16
10
2019
revised:
02
12
2019
accepted:
05
12
2019
pubmed:
7
2
2020
medline:
21
12
2021
entrez:
7
2
2020
Statut:
ppublish
Résumé
Acetylcholine (ACh) is known to regulate cortical activity during different behavioral states, for example, wakefulness and attention. Here we show a differential expression of muscarinic ACh receptors (mAChRs) and nicotinic ACh receptors (nAChRs) in different layer 6A (L6A) pyramidal cell (PC) types of somatosensory cortex. At low concentrations, ACh induced a persistent hyperpolarization in corticocortical (CC) but a depolarization in corticothalamic (CT) L6A PCs via M 4 and M1 mAChRs, respectively. At ~ 1 mM, ACh depolarized exclusively CT PCs via α4β2 subunit-containing nAChRs without affecting CC PCs. Miniature EPSC frequency in CC PCs was decreased by ACh but increased in CT PCs. In synaptic connections with a presynaptic CC PC, glutamate release was suppressed via M4 mAChR activation but enhanced by nAChRs via α4β2 nAChRs when the presynaptic neuron was a CT PC. Thus, in L6A, the interaction of mAChRs and nAChRs results in an altered excitability and synaptic release, effectively strengthening CT output while weakening CC synaptic signaling.
Identifiants
pubmed: 32026946
pii: 5685761
doi: 10.1093/cercor/bhz324
pmc: PMC7233001
doi:
Substances chimiques
Cholinergic Agonists
0
Receptor, Muscarinic M1
0
Receptor, Muscarinic M4
0
Receptors, Muscarinic
0
Receptors, Nicotinic
0
nicotinic receptor alpha4beta2
0
Glutamic Acid
3KX376GY7L
Acetylcholine
N9YNS0M02X
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3528-3542Informations de copyright
© The Author(s) 2019. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.
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