Optimizing Nervous System-Specific Gene Targeting with Cre Driver Lines: Prevalence of Germline Recombination and Influencing Factors.

Animals Female Gene Targeting / methods Genes, Reporter Germ Cells Integrases / genetics Male Mice Mice, Transgenic Mosaicism Neurons / metabolism Oocytes / metabolism Recombination, Genetic / genetics Spermatozoa / metabolism
Cre-lox conditional gene targeting conditional knockin conditional knockout conditional reporter germline recombination molecular genetics mosaic recombination parental sex bias site-specific recombinase

Journal

Neuron
ISSN: 1097-4199
Titre abrégé: Neuron
Pays: United States
ID NLM: 8809320

Informations de publication

Date de publication:
08 04 2020
Historique:
received: 25 07 2019
revised: 12 11 2019
accepted: 10 01 2020
pubmed: 7 2 2020
medline: 29 7 2020
entrez: 7 2 2020
Statut: ppublish

Résumé

The Cre-loxP system is invaluable for spatial and temporal control of gene knockout, knockin, and reporter expression in the mouse nervous system. However, we report varying probabilities of unexpected germline recombination in distinct Cre driver lines designed for nervous system-specific recombination. Selective maternal or paternal germline recombination is showcased with sample Cre lines. Collated data reveal germline recombination in over half of 64 commonly used Cre driver lines, in most cases with a parental sex bias related to Cre expression in sperm or oocytes. Slight differences among Cre driver lines utilizing common transcriptional control elements affect germline recombination rates. Specific target loci demonstrated differential recombination; thus, reporters are not reliable proxies for another locus of interest. Similar principles apply to other recombinase systems and other genetically targeted organisms. We hereby draw attention to the prevalence of germline recombination and provide guidelines to inform future research for the neuroscience and broader molecular genetics communities.

Identifiants

DOI: 10.1016/j.neuron.2020.01.008 PMID: 32027825 PMC: PMC7377387
pubmed: 32027825
pii: S0896-6273(20)30008-8
doi: 10.1016/j.neuron.2020.01.008
pmc: PMC7377387
mid: NIHMS1607662
pii:
doi:

Substances chimiques

Cre recombinase EC 2.7.7.-
Integrases EC 2.7.7.-

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, N.I.H., Intramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

37-65.e5

Subventions

Organisme : NIDA NIH HHS
ID : R00 DA041445
Pays : United States
Organisme : NEI NIH HHS
ID : P30 EY005722
Pays : United States
Organisme : NIDA NIH HHS
ID : F32 DA038913
Pays : United States
Organisme : NEI NIH HHS
ID : R01 EY030611
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS057819
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS102237
Pays : United States
Organisme : NIDA NIH HHS
ID : R01 DA031833
Pays : United States
Organisme : NICHD NIH HHS
ID : K99 HD092545
Pays : United States
Organisme : NIMH NIH HHS
ID : R37 MH049428
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS103484
Pays : United States
Organisme : NIDA NIH HHS
ID : K99 DA041445
Pays : United States
Organisme : NEI NIH HHS
ID : R01 EY024694
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS083898
Pays : United States
Organisme : NIMH NIH HHS
ID : R01 MH113349
Pays : United States
Organisme : NIDA NIH HHS
ID : R01 DA042744
Pays : United States
Organisme : NEI NIH HHS
ID : P30 EY012196
Pays : United States
Organisme : NIMH NIH HHS
ID : R01 MH111647
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS050835
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS099099
Pays : United States
Organisme : NIMH NIH HHS
ID : R01 MH081880
Pays : United States
Organisme : NICHD NIH HHS
ID : P50 HD103555
Pays : United States
Organisme : NINDS NIH HHS
ID : R37 NS029169
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS092578
Pays : United States
Organisme : Howard Hughes Medical Institute
Pays : United States
Organisme : NIMH NIH HHS
ID : R01 MH049428
Pays : United States
Organisme : NINDS NIH HHS
ID : F31 NS092419
Pays : United States

Informations de copyright

Copyright © 2020 Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of Interests The authors declare no competing interests.

Références

Neuron. 2009 Oct 29;64(2):227-39
pubmed: 19874790
Elife. 2016 Apr 15;5:
pubmed: 27083047
PLoS One. 2009;4(5):e5435
pubmed: 19415111
Nature. 2012 Mar 11;483(7390):465-9
pubmed: 22407321
Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):E1631-40
pubmed: 23569242
Nat Commun. 2019 May 22;10(1):2262
pubmed: 31118412
Cell Rep. 2019 Jan 29;26(5):1157-1173.e5
pubmed: 30699346
J Neurosci. 2012 May 30;32(22):7632-45
pubmed: 22649242
Annu Rev Neurosci. 2013 Jul 8;36:183-215
pubmed: 23682658
Elife. 2013 Dec 10;2:e01341
pubmed: 24327562
Genesis. 2006 Jan;44(1):44-9
pubmed: 16419044
Biochim Biophys Acta. 2007 Jun;1770(6):890-901
pubmed: 17363172
Genesis. 2000 Feb;26(2):130-2
pubmed: 10686607
J Neurosci. 2018 Mar 14;38(11):2713-2729
pubmed: 29439167
Biochem Biophys Res Commun. 1997 Aug 28;237(3):752-7
pubmed: 9299439
Science. 2008 Oct 31;322(5902):756-60
pubmed: 18974357
Front Synaptic Neurosci. 2017 Dec 19;9:17
pubmed: 29311892
Cell Metab. 2013 Jul 2;18(1):21-8
pubmed: 23823475
PLoS Biol. 2011 Nov;9(11):e1001194
pubmed: 22087075
Genesis. 2001 Oct;31(2):85-94
pubmed: 11668683
J Neurosci. 2006 Aug 16;26(33):8428-40
pubmed: 16914668
J Comp Neurol. 2008 Jan 1;506(1):16-29
pubmed: 17990269
Neuron. 2014 Jun 18;82(6):1317-33
pubmed: 24945774
Nucleic Acids Res. 2019 Jan 8;47(D1):D801-D806
pubmed: 30407599
Mol Endocrinol. 2001 Oct;15(10):1748-57
pubmed: 11579207
J Neurosci Methods. 2005 Apr 15;143(1):27-32
pubmed: 15763133
PLoS Biol. 2019 Oct 18;17(10):e3000492
pubmed: 31626642
Proc Natl Acad Sci U S A. 2008 May 13;105(19):6942-7
pubmed: 18460603
Nat Neurosci. 2010 Jan;13(1):133-40
pubmed: 20023653
Proc Natl Acad Sci U S A. 2000 Dec 5;97(25):13702-7
pubmed: 11087830
Cell. 2005 Nov 4;123(3):493-505
pubmed: 16269339
J Neurochem. 2017 Mar;140(5):787-798
pubmed: 27889925
Proc Natl Acad Sci U S A. 2002 Jun 11;99(12):8084-9
pubmed: 12060754
J Neurosci. 2015 Sep 16;35(37):12869-89
pubmed: 26377473
J Neurosci. 2000 Jan 15;20(2):709-21
pubmed: 10632600
Dis Model Mech. 2018 May 21;11(5):
pubmed: 29716894
PLoS One. 2011 Mar 10;6(3):e17611
pubmed: 21423695
Development. 2017 Mar 1;144(5):916-927
pubmed: 28174244
Mol Cell Neurosci. 2009 Feb;40(2):167-86
pubmed: 19026749
Am J Physiol Endocrinol Metab. 2005 Sep;289(3):E403-11
pubmed: 15870101
Nature. 2004 Jun 24;429(6994):900-3
pubmed: 15215868
J Neurosci. 2008 Dec 10;28(50):13435-47
pubmed: 19074017
Nature. 2017 Nov 8;551(7679):192-197
pubmed: 29120426
Proc Natl Acad Sci U S A. 1988 Jul;85(14):5166-70
pubmed: 2839833
J Neurosci. 2009 Feb 11;29(6):1874-86
pubmed: 19211894
Proc Natl Acad Sci U S A. 2007 Feb 13;104(7):2301-6
pubmed: 17284606
Cereb Cortex. 2009 Jul;19 Suppl 1:i1-10
pubmed: 19363146
Autophagy. 2007 Nov-Dec;3(6):591-6
pubmed: 17912025
Genesis. 2012 Jan;50(1):59-66
pubmed: 22223248
Biochem Biophys Res Commun. 2005 Jun 17;331(4):1216-21
pubmed: 15883005
Mol Cell Biol. 2009 Oct;29(19):5238-50
pubmed: 19635813
J Neurosci. 2014 Dec 10;34(50):16755-61
pubmed: 25505328
Genesis. 2013 Jun;51(6):436-42
pubmed: 23441020
J Neurosci. 2015 Nov 11;35(45):14983-99
pubmed: 26558771
Cell. 2012 Dec 7;151(6):1332-44
pubmed: 23217714
Neuron. 2011 Jul 14;71(1):142-54
pubmed: 21745644
J Neurosci. 1999 Oct 15;19(20):8954-65
pubmed: 10516314
Genesis. 2000 Nov-Dec;28(3-4):93-8
pubmed: 11105049
Neuron. 2018 Dec 5;100(5):1097-1115.e15
pubmed: 30392800
Neuron. 2016 Oct 5;92(1):59-74
pubmed: 27710791
Nature. 2005 Jul 21;436(7049):347-55
pubmed: 16034409
Neuron. 2019 Apr 3;102(1):105-119.e8
pubmed: 30792150
Mol Cell Biol. 2000 Jan;20(2):648-55
pubmed: 10611243
Elife. 2015 Jul 07;4:
pubmed: 26151762
Neuron. 1999 Oct;24(2):401-14
pubmed: 10571233
Nucleic Acids Res. 1995 Dec 25;23(24):5080-1
pubmed: 8559668
Eur J Neurosci. 2004 Aug;20(3):623-34
pubmed: 15255974
Endocrinology. 2008 May;149(5):2090-7
pubmed: 18276760
Proc Natl Acad Sci U S A. 2012 Oct 23;109(43):17651-6
pubmed: 23045697
Proc Natl Acad Sci U S A. 2018 Oct 16;115(42):10744-10749
pubmed: 30275311
PLoS One. 2013 Dec 09;8(12):e82818
pubmed: 24349371
Elife. 2019 Feb 08;8:
pubmed: 30735129
Development. 2006 Apr;133(8):1575-85
pubmed: 16556916
Mol Psychiatry. 2015 Jul;20(7):850-9
pubmed: 25824299
J Neurosci. 2006 Jan 25;26(4):1231-8
pubmed: 16436610
Vis Neurosci. 2011 Sep;28(5):381-92
pubmed: 21864449
Mol Psychiatry. 2018 May;23(5):1213-1225
pubmed: 28194005
Genesis. 2006 Dec;44(12):611-21
pubmed: 17146780
J Neurosci. 2012 Jan 25;32(4):1311-28
pubmed: 22279216
Neuron. 2015 Jan 21;85(2):316-29
pubmed: 25611509
Neuron. 2011 Sep 22;71(6):995-1013
pubmed: 21943598
Nature. 2006 Jun 15;441(7095):885-9
pubmed: 16625204
Genes Dev. 2000 Jun 1;14(11):1377-89
pubmed: 10837030
PLoS One. 2018 Feb 21;13(2):e0191436
pubmed: 29466446
Genes Dev. 1999 Dec 1;13(23):3136-48
pubmed: 10601039
Genesis. 2007 Sep;45(9):593-605
pubmed: 17868096
Science. 2002 Jul 12;297(5579):211-8
pubmed: 12040087
Nat Immunol. 2003 Mar;4(3):274-9
pubmed: 12563261
Genes Dev. 2001 Apr 1;15(7):859-76
pubmed: 11297510
Exp Mol Med. 2014 May 02;46:e93
pubmed: 24787734
Sci Data. 2018 Sep 11;5:180192
pubmed: 30204153
Front Neural Circuits. 2014 Jul 10;8:76
pubmed: 25071457
Mol Metab. 2018 Aug;14:130-138
pubmed: 29914853
Glia. 2020 Jan;68(1):161-177
pubmed: 31453649
Neuron. 2015 May 20;86(4):1091-1099
pubmed: 25996136
Proc Natl Acad Sci U S A. 2011 Jun 14;108(24):9987-92
pubmed: 21628556
Proc Natl Acad Sci U S A. 2011 Dec 27;108(52):E1475-83
pubmed: 22123957
eNeuro. 2017 Sep 6;4(5):
pubmed: 28932809
Science. 2012 Mar 16;335(6074):1373-6
pubmed: 22345400
Cell Metab. 2011 Feb 2;13(2):195-204
pubmed: 21284986
Cell. 2018 Feb 8;172(4):706-718.e15
pubmed: 29398114
Genesis. 2006 Aug;44(8):355-60
pubmed: 16847871
Nat Neurosci. 1999 Feb;2(2):115-7
pubmed: 10195193
Cell. 1996 Dec 27;87(7):1327-38
pubmed: 8980238
Nat Methods. 2005 Jun;2(6):419-26
pubmed: 15908920
Brain. 2018 May 1;141(5):1350-1374
pubmed: 29538625
Curr Biol. 1998 Dec 3;8(24):1323-6
pubmed: 9843687
Genesis. 2003 Sep;37(1):44-50
pubmed: 14502577
Nat Neurosci. 2005 Apr;8(4):468-75
pubmed: 15793579
Nat Genet. 1999 Sep;23(1):99-103
pubmed: 10471508
PLoS One. 2016 Feb 17;11(2):e0149379
pubmed: 26886559
Cell Metab. 2011 May 4;13(5):584-91
pubmed: 21531340
Genesis. 2007 Jun;45(6):413-7
pubmed: 17551945
Cell Rep. 2018 Jun 19;23(12):3480-3491
pubmed: 29924992
Hum Mol Genet. 2011 Jul 1;20(13):2642-50
pubmed: 21505076
Nat Neurosci. 2016 Jan;19(1):65-74
pubmed: 26656646
Mol Vis. 2008 Mar 20;14:562-71
pubmed: 18385792
Mol Cell Biol. 2003 Feb;23(3):1044-53
pubmed: 12529408
Neuron. 2010 Mar 25;65(6):886-98
pubmed: 20346763
Dev Biol. 2000 Jun 15;222(2):296-306
pubmed: 10837119
Genesis. 2000 Feb;26(2):133-5
pubmed: 10686608
Mol Vis. 2016 Jun 23;22:705-17
pubmed: 27390513
EMBO Rep. 2000 Aug;1(2):133-9
pubmed: 11265752
Neuron. 2016 Oct 19;92(2):392-406
pubmed: 27693255
Nat Genet. 1999 Jan;21(1):70-1
pubmed: 9916792
J Mol Biol. 1981 Aug 25;150(4):467-86
pubmed: 6276557
Neuron. 2016 May 18;90(4):752-67
pubmed: 27133466
Neuron. 2011 Dec 8;72(5):721-33
pubmed: 22153370
Cell. 2018 Jul 12;174(2):465-480.e22
pubmed: 30007418
Front Neural Circuits. 2018 Sep 11;12:68
pubmed: 30254571
Genesis. 2006 Aug;44(8):383-90
pubmed: 16865686
Mamm Genome. 2012 Oct;23(9-10):587-99
pubmed: 22926223
Neuron. 2008 Aug 14;59(3):497-508
pubmed: 18701074
Am J Pathol. 2010 Jan;176(1):85-97
pubmed: 20008127
Nature. 2003 Oct 30;425(6961):917-25
pubmed: 14586460
Genesis. 2006 Jul;44(7):328-35
pubmed: 16823860
Neuron. 2018 Sep 5;99(5):985-998.e6
pubmed: 30122381
PLoS One. 2011;6(8):e23279
pubmed: 21876744
Nature. 2010 Nov 11;468(7321):263-9
pubmed: 21068835
J Neurosci. 2009 Sep 2;29(35):10869-82
pubmed: 19726645
Nat Rev Genet. 2008 Oct;9(10):803-10
pubmed: 18781157
J Neurosci. 2007 Sep 12;27(37):9817-23
pubmed: 17855595
Hear Res. 2014 Mar;309:124-35
pubmed: 24333301
Cell. 2017 May 4;169(4):621-635.e16
pubmed: 28475893
J Neurosci. 2002 Aug 1;22(15):6309-14
pubmed: 12151506
Dev Cell. 2009 Dec;17(6):861-73
pubmed: 20059955
Development. 2016 May 1;143(9):1534-46
pubmed: 27143755
Nature. 2012 Aug 30;488(7413):647-51
pubmed: 22763451
Trends Genet. 2018 May;34(5):333-340
pubmed: 29336844
Elife. 2017 Jun 13;6:
pubmed: 28608780
Science. 1994 Jul 1;265(5168):103-6
pubmed: 8016642
Science. 2012 Aug 10;337(6095):746-9
pubmed: 22879516
Mol Pharmacol. 2008 Feb;73(2):282-91
pubmed: 17965197
Proc Natl Acad Sci U S A. 2009 Aug 18;106(33):13939-44
pubmed: 19666516
Cell. 1996 Dec 27;87(7):1317-26
pubmed: 8980237
Neuron. 2013 Oct 2;80(1):113-28
pubmed: 24094106
Neuron. 2013 Dec 18;80(6):1368-83
pubmed: 24360541
PLoS One. 2011;6(11):e28137
pubmed: 22140524
Nat Neurosci. 2008 Jun;11(6):721-8
pubmed: 18454144
Nat Neurosci. 2012 Mar 25;15(5):793-802
pubmed: 22446880
Neural Dev. 2019 Feb 14;14(1):4
pubmed: 30764875
Front Neural Circuits. 2013 Oct 16;7:168
pubmed: 24137112
Nat Neurosci. 2013 Nov;16(11):1598-607
pubmed: 24097043
PLoS Biol. 2005 May;3(5):e159
pubmed: 15836427
Proc Natl Acad Sci U S A. 1996 Jun 11;93(12):5860-5
pubmed: 8650183
J Biol Chem. 2003 Oct 24;278(43):42313-20
pubmed: 12896967
Sci Rep. 2018 Apr 25;8(1):6521
pubmed: 29695820
Nat Commun. 2012;3:1218
pubmed: 23169059
Cereb Cortex. 2017 Jul 1;27(7):3553-3567
pubmed: 27312991
Annu Rev Neurosci. 2018 Jul 8;41:323-348
pubmed: 29709207
Cell. 2011 Jan 21;144(2):282-95
pubmed: 21241895
Elife. 2018 Apr 03;7:
pubmed: 29611808
J Neurosci. 2010 Mar 10;30(10):3803-12
pubmed: 20220015

Auteurs

Lin Luo (L)

Djavad Mowafaghian Centre for Brain Health and Department of Psychiatry, University of British Columbia, 2211 Wesbrook Mall, Vancouver, BC V6T 2B5, Canada.

Mateusz C Ambrozkiewicz (MC)

Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, Hermann-Rein-Strasse 3, 37075 Göttingen, Germany; Institute of Cell Biology and Neurobiology, Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Charitéplatz 1, 10117 Berlin, Germany.

Fritz Benseler (F)

Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, Hermann-Rein-Strasse 3, 37075 Göttingen, Germany.

Cui Chen (C)

State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science, Institutes of Brain Science, Fudan University, Shanghai 200032, China.

Emilie Dumontier (E)

Department of Neurology & Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, QC H3A 2B4, Canada.

Susanne Falkner (S)

Biozentrum of the University of Basel, Basel, Switzerland.

Elisabetta Furlanis (E)

Biozentrum of the University of Basel, Basel, Switzerland.

Andrea M Gomez (AM)

Biozentrum of the University of Basel, Basel, Switzerland.

Naosuke Hoshina (N)

F.M. Kirby Neurobiology Center, Department of Neurology, Boston Children's Hospital, Harvard Medical School, Boston, MA 02115, USA.

Wei-Hsiang Huang (WH)

Department of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA; Centre for Research in Neuroscience, Department of Neurology and Neurosurgery, The Research Institute of the McGill University Health Centre, Montreal, QC H3G 1A4, Canada.

Mary Anne Hutchison (MA)

Synapse and Neural Circuit Research Unit, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA.

Yu Itoh-Maruoka (Y)

Division of Pathogenetic Signaling, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, 1-5-6 Minatojima-minamimachi, Chuo-ku, Kobe, Hyogo 650-0047, Japan.

Laura A Lavery (LA)

Department of Molecular and Human Genetics, Jan and Dan Duncan Neurological Research Institute at Texas Children's Hospital, Houston, TX 77003, USA; Howard Hughes Medical Institute, Baylor College of Medicine, Houston, TX 77030, USA.

Wei Li (W)

State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science, Institutes of Brain Science, Fudan University, Shanghai 200032, China.

Tomohiko Maruo (T)

Division of Pathogenetic Signaling, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, 1-5-6 Minatojima-minamimachi, Chuo-ku, Kobe, Hyogo 650-0047, Japan; Department of Biochemistry, Tokushima University Graduate School of Medical Sciences, 3-18-15, Kuramoto-cho, Tokushima 770-8503, Japan; Department of Biochemistry, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami-ku, Sagamihara, Kanagawa 252-0374, Japan.

Junko Motohashi (J)

Department of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan.

Emily Ling-Lin Pai (EL)

Nina Ireland Laboratory of Developmental Neurobiology, Department of Psychiatry, UCSF Weill Institute for Neurosciences, University of California, San Francisco, San Francisco, CA 94158, USA; Neuroscience Graduate Program, University of California, San Francisco, San Francisco, CA 94158, USA.

Kenneth A Pelkey (KA)

Section on Cellular and Synaptic Physiology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA.

Ariane Pereira (A)

Department of Neurobiology and Department of Ophthalmology, Duke University School of Medicine, Durham, NC 27710, USA.

Thomas Philips (T)

Department of Neurology and Brain Science Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.

Jennifer L Sinclair (JL)

Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, Bethesda, MD 20892, USA.

Jeff A Stogsdill (JA)

Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA; Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA 02139, USA.

Lisa Traunmüller (L)

Biozentrum of the University of Basel, Basel, Switzerland.

Jiexin Wang (J)

Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA.

Joke Wortel (J)

Department of Functional Genomics and Department of Clinical Genetics, Center for Neurogenomics and Cognitive Research (CNCR), VU University Amsterdam and University Medical Center Amsterdam, de Boelelaan 1085, 1081 HV Amsterdam, the Netherlands.

Wenjia You (W)

Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA; Departments of Genetics, Harvard Medical School, Boston, MA 02115, USA; Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02115, USA.

Nashat Abumaria (N)

State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science, Institutes of Brain Science, Fudan University, Shanghai 200032, China; Department of Laboratory Animal Science, Shanghai Medical College, Fudan University, Shanghai 200032, China.

Kevin T Beier (KT)

Department of Physiology and Biophysics, Center for the Neurobiology of Learning and Memory, University of California, Irvine, Irvine, CA 92697, USA.

Nils Brose (N)

Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, Hermann-Rein-Strasse 3, 37075 Göttingen, Germany.

Harold A Burgess (HA)

Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, Bethesda, MD 20892, USA.

Constance L Cepko (CL)

Departments of Genetics, Harvard Medical School, Boston, MA 02115, USA; Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02115, USA.

Jean-François Cloutier (JF)

Department of Neurology & Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, QC H3A 2B4, Canada.

Cagla Eroglu (C)

Department of Cell Biology, Department of Neurobiology, and Duke Institute for Brain Sciences, Regeneration Next Initiative, Duke University Medical Center, Durham, NC 27710, USA.

Sandra Goebbels (S)

Department of Neurogenetics, Max Planck Institute of Experimental Medicine, Hermann-Rein-Strasse 3, 37075 Göttingen, Germany.

Pascal S Kaeser (PS)

Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA.

Jeremy N Kay (JN)

Department of Neurobiology and Department of Ophthalmology, Duke University School of Medicine, Durham, NC 27710, USA.

Wei Lu (W)

Synapse and Neural Circuit Research Unit, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA.

Liqun Luo (L)

Department of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.

Kenji Mandai (K)

Division of Pathogenetic Signaling, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, 1-5-6 Minatojima-minamimachi, Chuo-ku, Kobe, Hyogo 650-0047, Japan; Department of Biochemistry, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami-ku, Sagamihara, Kanagawa 252-0374, Japan.

Chris J McBain (CJ)

Section on Cellular and Synaptic Physiology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA.

Klaus-Armin Nave (KA)

Department of Neurogenetics, Max Planck Institute of Experimental Medicine, Hermann-Rein-Strasse 3, 37075 Göttingen, Germany.

Marco A M Prado (MAM)

Robarts Research Institute, Department of Anatomy and Cell Biology, and Department of Physiology and Pharmacology, Schulich School of Medicine & Dentistry, University of Western Ontario, London, ON N6A 5B7, Canada; Brain and Mind Institute, The University of Western Ontario, London, ON N6A 5B7, Canada.

Vania F Prado (VF)

Robarts Research Institute, Department of Anatomy and Cell Biology, and Department of Physiology and Pharmacology, Schulich School of Medicine & Dentistry, University of Western Ontario, London, ON N6A 5B7, Canada; Brain and Mind Institute, The University of Western Ontario, London, ON N6A 5B7, Canada.

Jeffrey Rothstein (J)

Department of Neurology and Brain Science Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.

John L R Rubenstein (JLR)

Nina Ireland Laboratory of Developmental Neurobiology, Department of Psychiatry, UCSF Weill Institute for Neurosciences, University of California, San Francisco, San Francisco, CA 94158, USA; Neuroscience Graduate Program, University of California, San Francisco, San Francisco, CA 94158, USA.

Gesine Saher (G)

Department of Neurogenetics, Max Planck Institute of Experimental Medicine, Hermann-Rein-Strasse 3, 37075 Göttingen, Germany.

Kenji Sakimura (K)

Department of Cellular Neurobiology, Brain Research Institute, Niigata University, Niigata 951-8585, Japan.

Joshua R Sanes (JR)

Center for Brain Science and Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA.

Peter Scheiffele (P)

Biozentrum of the University of Basel, Basel, Switzerland.

Yoshimi Takai (Y)

Division of Pathogenetic Signaling, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, 1-5-6 Minatojima-minamimachi, Chuo-ku, Kobe, Hyogo 650-0047, Japan.

Hisashi Umemori (H)

F.M. Kirby Neurobiology Center, Department of Neurology, Boston Children's Hospital, Harvard Medical School, Boston, MA 02115, USA.

Matthijs Verhage (M)

Department of Functional Genomics and Department of Clinical Genetics, Center for Neurogenomics and Cognitive Research (CNCR), VU University Amsterdam and University Medical Center Amsterdam, de Boelelaan 1085, 1081 HV Amsterdam, the Netherlands.

Michisuke Yuzaki (M)

Department of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan.

Huda Yahya Zoghbi (HY)

Department of Molecular and Human Genetics, Jan and Dan Duncan Neurological Research Institute at Texas Children's Hospital, Houston, TX 77003, USA; Howard Hughes Medical Institute, Baylor College of Medicine, Houston, TX 77030, USA.

Hiroshi Kawabe (H)

Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, Hermann-Rein-Strasse 3, 37075 Göttingen, Germany; Division of Pathogenetic Signaling, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, 1-5-6 Minatojima-minamimachi, Chuo-ku, Kobe, Hyogo 650-0047, Japan; Department of Gerontology, Laboratory of Molecular Life Science, Institute of Biomedical Research and Innovation, Foundation for Biomedical Research and Innovation at Kobe, 2-2 Minatojima-minamimachi Chuo-ku, Kobe, Hyogo 650-0047, Japan. Electronic address: kawabe@em.mpg.de.

Ann Marie Craig (AM)

Djavad Mowafaghian Centre for Brain Health and Department of Psychiatry, University of British Columbia, 2211 Wesbrook Mall, Vancouver, BC V6T 2B5, Canada. Electronic address: acraig@mail.ubc.ca.

Articles similaires

Smoking Cessation and Incident Cardiovascular Disease.

Jun Hwan Cho, Seung Yong Shin, Hoseob Kim et al.
1.00
Humans Male Smoking Cessation Cardiovascular Diseases Female

Evaluation of Low-Value Services Across Major Medicare Advantage Insurers and Traditional Medicare.

Ciara Duggan, Adam L Beckman, Ishani Ganguli et al.
1.00
Humans United States Aged Cross-Sectional Studies Medicare Part C

Effectiveness of Virtual Yoga for Chronic Low Back Pain: A Randomized Clinical Trial.

Hallie Tankha, Devyn Gaskins, Amanda Shallcross et al.
1.00
Humans Yoga Low Back Pain Female Male

Meal Timing and Anthropometric and Metabolic Outcomes: A Systematic Review and Meta-Analysis.

Hiu Yee Liu, Ashley A Eso, Nathan Cook et al.
1.00
Humans Meals Time Factors Female Adult

Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Optimizing Nervous System-Specific Gene...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Ciblage de gène › Optimizing Nervous System-Specific Gene...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Composants de génome › Gènes › Gènes rapporteurs › Optimizing Nervous System-Specific Gene...
questionsmedicales.fr › Cellules › Cellules germinales › Optimizing Nervous System-Specific Gene...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Recombinases › Integrases › Optimizing Nervous System-Specific Gene...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Optimizing Nervous System-Specific Gene...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Souris transgéniques › Optimizing Nervous System-Specific Gene...
questionsmedicales.fr › Phénomènes génétiques › Variation génétique › Mutation › Aberrations des chromosomes › Mosaïcisme › Optimizing Nervous System-Specific Gene...
questionsmedicales.fr › Cellules › Neurones › Optimizing Nervous System-Specific Gene...
questionsmedicales.fr › Cellules › Cellules germinales › Ovocytes › Optimizing Nervous System-Specific Gene...
questionsmedicales.fr › Phénomènes génétiques › Recombinaison génétique › Optimizing Nervous System-Specific Gene...
questionsmedicales.fr › Cellules › Cellules germinales › Spermatozoïdes › Optimizing Nervous System-Specific Gene...