Skin surface Protoporphyrin IX fluorescence is associated with epidermal but not dermal fluorescence intensities.
Hexyl aminolevulinate
Methyl aminolevulinate
PpIX fluorescence
PpIX fluorescence microscopy
PpIX fluorescence photographs
Journal
Photodiagnosis and photodynamic therapy
ISSN: 1873-1597
Titre abrégé: Photodiagnosis Photodyn Ther
Pays: Netherlands
ID NLM: 101226123
Informations de publication
Date de publication:
Jun 2020
Jun 2020
Historique:
received:
14
08
2019
revised:
17
01
2020
accepted:
31
01
2020
pubmed:
8
2
2020
medline:
15
5
2021
entrez:
8
2
2020
Statut:
ppublish
Résumé
Photodynamic therapy with Protoporphyrin IX (PpIX) is well-established for keratinocyte dysplasia. PpIX fluorescence can be quantified at the skin surface and within skin layers, but their relation is previously undescribed. The study objective was to assess the relation between skin surface PpIX fluorescence and PpIX fluorescence in epidermis and dermis. Normal buttocks skin was tape-stripped and incubated with 20 % methyl aminolevulinate cream and 20 % hexyl aminolevulinate cream under occlusion. After 3 h, skin surface PpIX fluorescence photographs were captured and 4 mm punch biopsies sampled. PpIX fluorescence microscopy was measured in regions of interest (ROI) in epidermis and superficial dermis. All PpIX fluorescence intensities were calculated in arbitrary units (AU). Skin surface PpIX fluorescence intensity expressed a positive, linear relation to ROI-epidermis PpIX fluorescence (HAL- and MAL-incubated skin, p < 0.001, r The positive linear relation between skin surface PpIX fluorescence photographs and epidermal PpIX fluorescence microscopy indicates that skin surface PpIX fluorescence predominantly derives from epidermis.
Sections du résumé
BACKGROUND
BACKGROUND
Photodynamic therapy with Protoporphyrin IX (PpIX) is well-established for keratinocyte dysplasia. PpIX fluorescence can be quantified at the skin surface and within skin layers, but their relation is previously undescribed. The study objective was to assess the relation between skin surface PpIX fluorescence and PpIX fluorescence in epidermis and dermis.
METHODS
METHODS
Normal buttocks skin was tape-stripped and incubated with 20 % methyl aminolevulinate cream and 20 % hexyl aminolevulinate cream under occlusion. After 3 h, skin surface PpIX fluorescence photographs were captured and 4 mm punch biopsies sampled. PpIX fluorescence microscopy was measured in regions of interest (ROI) in epidermis and superficial dermis. All PpIX fluorescence intensities were calculated in arbitrary units (AU).
RESULTS
RESULTS
Skin surface PpIX fluorescence intensity expressed a positive, linear relation to ROI-epidermis PpIX fluorescence (HAL- and MAL-incubated skin, p < 0.001, r
CONCLUSION
CONCLUSIONS
The positive linear relation between skin surface PpIX fluorescence photographs and epidermal PpIX fluorescence microscopy indicates that skin surface PpIX fluorescence predominantly derives from epidermis.
Identifiants
pubmed: 32032781
pii: S1572-1000(20)30034-X
doi: 10.1016/j.pdpdt.2020.101681
pii:
doi:
Substances chimiques
Photosensitizing Agents
0
Protoporphyrins
0
Aminolevulinic Acid
88755TAZ87
protoporphyrin IX
C2K325S808
Types de publication
Case Reports
Langues
eng
Sous-ensembles de citation
IM
Pagination
101681Informations de copyright
Copyright © 2020. Published by Elsevier B.V.
Déclaration de conflit d'intérêts
Declaration of Competing Interest CF, HCW, KTB, MH and PAP have no conflict of interest. HAL powder was provided by Photocure.