Release of antidiabetic peptides from Stichopus japonicas by simulated gastrointestinal digestion.
3T3-L1 Cells
Animals
Chromatography, Liquid
Digestion
Dipeptidyl Peptidase 4
/ chemistry
Dipeptidyl-Peptidase IV Inhibitors
/ chemistry
Hep G2 Cells
Humans
Hydrolysis
Hypoglycemic Agents
/ metabolism
Mice
Molecular Docking Simulation
Peptides
/ analysis
Stichopus
/ chemistry
Tandem Mass Spectrometry
Antidiabetic activity
DPP-IV
Gastrointestinal digestion
LC–MS/MS
Molecular docking
Sea cucumber
Tricine-SDS-PAGE
Journal
Food chemistry
ISSN: 1873-7072
Titre abrégé: Food Chem
Pays: England
ID NLM: 7702639
Informations de publication
Date de publication:
15 Jun 2020
15 Jun 2020
Historique:
received:
27
08
2019
revised:
29
12
2019
accepted:
19
01
2020
pubmed:
8
2
2020
medline:
25
4
2020
entrez:
8
2
2020
Statut:
ppublish
Résumé
Sea cucumber (Stichopus japonicus) is a high-protein food with the potential to release certain peptides through enzymolysis. This work is to explore the characteristics of peptides released from Stichopus japonicus protein in the process of digestion. Hydrolysates were obtained by gastrointestinal digestion and fractioned to <3, 3-10, 10-30 and >30 kDa fractions. Fifty-eight peptides from <3 kDa fraction were characterized using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Hydrolysates could improve glucose uptake of 3 T3-L1 cells and high insulin-induced insulin-resistant Hep G2 cells. Molecular docking showed that the released peptides had similar binding mode with anagliptin, a dipeptidyl peptidase IV (DPP-IV) inhibitor. The <3 kDa fraction in gastro and intestinal digestion showed the greatest DPP-IV inhibitory potency (IC
Identifiants
pubmed: 32032832
pii: S0308-8146(20)30121-7
doi: 10.1016/j.foodchem.2020.126273
pii:
doi:
Substances chimiques
Dipeptidyl-Peptidase IV Inhibitors
0
Hypoglycemic Agents
0
Peptides
0
DPP4 protein, human
EC 3.4.14.5
Dipeptidyl Peptidase 4
EC 3.4.14.5
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
126273Informations de copyright
Copyright © 2020 Elsevier Ltd. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.