Structural Dynamics of Lytic Polysaccharide Monooxygenase during Catalysis.
Catalysis
Catalytic Domain
Cellulose
/ chemistry
Copper
/ chemistry
Fungal Proteins
/ chemistry
Hydrogen-Ion Concentration
Lignin
/ chemistry
Mass Spectrometry
Mixed Function Oxygenases
/ chemistry
Neurospora crassa
/ enzymology
Oxidative Stress
Oxidoreductases
/ chemistry
Oxygen
/ chemistry
Polysaccharides
/ chemistry
Protein Binding
Protein Conformation
Reactive Oxygen Species
/ chemistry
Spectrometry, Mass, Electrospray Ionization
Substrate Specificity
hydrogen/deuterium exchange mass spectrometry
lignocellulose degradation
lytic polysaccharide monooxygenase
oxidative amino acid modification
peptide bond cleavage
reactive oxygen species
Journal
Biomolecules
ISSN: 2218-273X
Titre abrégé: Biomolecules
Pays: Switzerland
ID NLM: 101596414
Informations de publication
Date de publication:
05 02 2020
05 02 2020
Historique:
received:
15
01
2020
revised:
30
01
2020
accepted:
31
01
2020
entrez:
9
2
2020
pubmed:
9
2
2020
medline:
30
3
2021
Statut:
epublish
Résumé
Lytic polysaccharide monooxygenases (LPMOs) are industrially important oxidoreductases employed in lignocellulose saccharification. Using advanced time-resolved mass spectrometric techniques, we elucidated the structural determinants for substrate-mediated stabilization of the fungal LPMO9C from
Identifiants
pubmed: 32033404
pii: biom10020242
doi: 10.3390/biom10020242
pmc: PMC7072406
pii:
doi:
Substances chimiques
Fungal Proteins
0
Polysaccharides
0
Reactive Oxygen Species
0
lignocellulose
11132-73-3
Copper
789U1901C5
Cellulose
9004-34-6
Lignin
9005-53-2
Mixed Function Oxygenases
EC 1.-
Oxidoreductases
EC 1.-
Oxygen
S88TT14065
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Ministry of Education, Youth and Science
ID : LM2015043
Pays : International
Organisme : Austrian Science Fund
ID : W1224
Pays : International
Organisme : Austrian Science Fund
ID : J-4154
Pays : International
Organisme : Grantová Agentura České Republiky
ID : 16-34818L
Pays : International
Organisme : Austrian Science Fund
ID : I 2385-N28
Pays : International
Organisme : Ministry of Education, Youth and Science
ID : LQ1604
Pays : International
Organisme : Austrian Science Fund FWF
ID : W 1224
Pays : Austria
Organisme : European Regional Development Fund
ID : CZ.1.05/1.1.00/02.0109
Pays : International
Organisme : Univerzita Karlova v Praze
ID : SVV260427/2019
Pays : International
Déclaration de conflit d'intérêts
The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.
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