Application of the dynamic gastrointestinal simulator (simgi®) to assess the impact of probiotic supplementation in the metabolism of grape polyphenols.
Adult
Ammonium Compounds
/ metabolism
Benzoates
/ metabolism
Biflavonoids
/ analysis
Catechin
/ analysis
Colony Count, Microbial
DNA, Bacterial
/ genetics
Diet, Western
Female
Gastrointestinal Microbiome
Gastrointestinal Tract
/ metabolism
Healthy Volunteers
Humans
Lactobacillus plantarum
/ metabolism
Molecular Weight
Polyphenols
/ analysis
Proanthocyanidins
/ analysis
Probiotics
RNA, Ribosomal, 16S
/ genetics
Sequence Analysis, DNA
Vitis
/ chemistry
Gut microbiota
Lactobacillus plantarum CLC 17
Metabolism
Phenolic metabolites
Simgi® model
Journal
Food research international (Ottawa, Ont.)
ISSN: 1873-7145
Titre abrégé: Food Res Int
Pays: Canada
ID NLM: 9210143
Informations de publication
Date de publication:
03 2020
03 2020
Historique:
received:
25
08
2019
revised:
28
10
2019
accepted:
30
10
2019
entrez:
11
2
2020
pubmed:
11
2
2020
medline:
9
3
2021
Statut:
ppublish
Résumé
In this paper, the Dynamic Gastrointestinal Simulator (simgi®) is used as a model to the study the metabolic activity of probiotics at the intestinal level, and in particular, to assess the impact of probiotic supplementation in the microbial metabolism of grape polyphenols. Two independent simulations using fecal samples from two healthy volunteers were carried out. Changes in microbiota composition and in metabolic activity were assessed by qPCR and 16S rRNA gene sequencing and by analyses of phenolic metabolites and ammonium ions (NH
Identifiants
pubmed: 32036893
pii: S0963-9969(19)30676-3
doi: 10.1016/j.foodres.2019.108790
pii:
doi:
Substances chimiques
Ammonium Compounds
0
Benzoates
0
Biflavonoids
0
DNA, Bacterial
0
Polyphenols
0
Proanthocyanidins
0
RNA, Ribosomal, 16S
0
procyanidin
4852-22-6
Catechin
8R1V1STN48
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
108790Informations de copyright
Copyright © 2019 Elsevier Ltd. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest We declare that we have no conflict of interest.