Targeted nanopore sequencing with Cas9-guided adapter ligation.


Journal

Nature biotechnology
ISSN: 1546-1696
Titre abrégé: Nat Biotechnol
Pays: United States
ID NLM: 9604648

Informations de publication

Date de publication:
04 2020
Historique:
received: 04 06 2019
accepted: 06 01 2020
pubmed: 12 2 2020
medline: 9 7 2020
entrez: 12 2 2020
Statut: ppublish

Résumé

Despite recent improvements in sequencing methods, there remains a need for assays that provide high sequencing depth and comprehensive variant detection. Current methods

Identifiants

pubmed: 32042167
doi: 10.1038/s41587-020-0407-5
pii: 10.1038/s41587-020-0407-5
pmc: PMC7145730
mid: NIHMS1548342
doi:

Substances chimiques

RNA, Guide 0
CRISPR-Associated Protein 9 EC 3.1.-

Types de publication

Journal Article Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

IM

Pagination

433-438

Subventions

Organisme : NCI NIH HHS
ID : T32 CA130840
Pays : United States
Organisme : NCI NIH HHS
ID : T32 CA153952
Pays : United States
Organisme : NHGRI NIH HHS
ID : R01 HG009190
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM007057
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM136577
Pays : United States

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Auteurs

Timothy Gilpatrick (T)

Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, USA.

Isac Lee (I)

Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, USA.

James E Graham (JE)

Oxford Nanopore Technologies, Oxford, UK.

Etienne Raimondeau (E)

Oxford Nanopore Technologies, Oxford, UK.

Rebecca Bowen (R)

Oxford Nanopore Technologies, Oxford, UK.

Andrew Heron (A)

Oxford Nanopore Technologies, Oxford, UK.

Bradley Downs (B)

Department of Oncology, Johns Hopkins School of Medicine, Baltimore, MD, USA.

Saraswati Sukumar (S)

Department of Oncology, Johns Hopkins School of Medicine, Baltimore, MD, USA.

Fritz J Sedlazeck (FJ)

Human Genome Sequencing Center, Baylor College of Medicine, Houston, TX, USA.

Winston Timp (W)

Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, USA. wtimp@jhu.edu.
Department of Molecular Biology and Genetics, Department of Medicine, Division of Infectious Disease, Johns Hopkins School of Medicine, Baltimore, MD, USA. wtimp@jhu.edu.

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Classifications MeSH