Flexibility of the petunia strigolactone receptor DAD2 promotes its interaction with signaling partners.
F-Box Proteins
/ chemistry
Gene Expression Regulation, Plant
/ genetics
Heterocyclic Compounds, 3-Ring
/ chemistry
Hydrolases
/ chemistry
Lactones
/ chemistry
Petunia
/ chemistry
Plant Growth Regulators
/ chemistry
Plant Proteins
/ chemistry
Protein Binding
/ genetics
SKP Cullin F-Box Protein Ligases
/ chemistry
Signal Transduction
/ genetics
DAD2
Petunia hybrida
crystallography
molecular dynamics
mutagenesis
phytohormone signaling
plant hormone
receptor
signal transduction
strigolactone
Journal
The Journal of biological chemistry
ISSN: 1083-351X
Titre abrégé: J Biol Chem
Pays: United States
ID NLM: 2985121R
Informations de publication
Date de publication:
27 03 2020
27 03 2020
Historique:
received:
16
10
2019
revised:
13
02
2020
pubmed:
20
2
2020
medline:
15
12
2020
entrez:
20
2
2020
Statut:
ppublish
Résumé
Strigolactones (SLs) are terpenoid-derived plant hormones that regulate various developmental processes, particularly shoot branching, root development, and leaf senescence. The SL receptor has an unusual mode of action. Upon binding SL, it hydrolyzes the hormone, and then covalently binds one of the hydrolytic products. These initial events enable the SL receptor DAD2 (in petunia) to interact with the F-box protein PhMAX2A of the Skp-Cullin-F-box (SCF) complex and/or a repressor of SL signaling, PhD53A. However, it remains unclear how binding and hydrolysis structurally alters the SL receptor to enable its engagement with signaling partners. Here, we used mutagenesis to alter DAD2 and affect SL hydrolysis or DAD2's ability to interact with its signaling partners. We identified three DAD2 variants whose hydrolytic activity had been separated from the receptor's interactions with PhMAX2A or PhD53A. Two variants, DAD2
Identifiants
pubmed: 32071083
pii: S0021-9258(17)48746-1
doi: 10.1074/jbc.RA119.011509
pmc: PMC7105320
pii:
doi:
Substances chimiques
F-Box Proteins
0
GR24 strigolactone
0
Heterocyclic Compounds, 3-Ring
0
Lactones
0
Plant Growth Regulators
0
Plant Proteins
0
SKP Cullin F-Box Protein Ligases
EC 2.3.2.27
Hydrolases
EC 3.-
Banques de données
PDB
['4DNP', '6UH8', '6UH9']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
4181-4193Informations de copyright
© 2020 Lee et al.
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