DNA 5-Methylcytosine-Specific Amplification and Sequencing.

5-Methylcytosine / chemistry Animals DNA / analysis DNA Methylation Humans Mice Nucleic Acid Amplification Techniques / methods Sequence Analysis, DNA / methods Sulfites / chemistry Whole Genome Sequencing

Journal

Journal of the American Chemical Society
ISSN: 1520-5126
Titre abrégé: J Am Chem Soc
Pays: United States
ID NLM: 7503056

Informations de publication

Date de publication:
11 03 2020
Historique:
pubmed: 23 2 2020
medline: 14 5 2021
entrez: 21 2 2020
Statut: ppublish

Résumé

DNA 5-methylcytosine (5mC)-specific mapping has been hampered by severe DNA degradation and the presence of 5-hydroxymethylcytosine (5hmC) using the conventional bisulfite sequencing approach. Here, we present a 5mC-specific whole-genome amplification method (5mC-WGA), with which we achieved 5mC retention during DNA amplification from limited input down to 10 pg scale with limited interference from 5hmC signals, providing DNA 5mC methylome with high reproducibility and accuracy.

Identifiants

DOI: 10.1021/jacs.9b12707 PMID: 32077696 PMC: PMC7216556
pubmed: 32077696
doi: 10.1021/jacs.9b12707
pmc: PMC7216556
mid: NIHMS1580469
doi:

Substances chimiques

Sulfites 0
5-Methylcytosine 6R795CQT4H
DNA 9007-49-2
hydrogen sulfite OJ9787WBLU

Types de publication

Journal Article Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

IM

Pagination

4539-4543

Subventions

Organisme : NHGRI NIH HHS
ID : RM1 HG008935
Pays : United States

Commentaires et corrections

Type : ErratumIn

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Auteurs

Chang Liu (C)

Department of Chemistry, Department of Biochemistry and Molecular Biology, and Institute for Biophysical Dynamics, The University of Chicago, Chicago, Illinois 60637, United States.
Howard Hughes Medical Institute, The University of Chicago, Chicago, Illinois 60637, United States.

Xiaolong Cui (X)

Department of Chemistry, Department of Biochemistry and Molecular Biology, and Institute for Biophysical Dynamics, The University of Chicago, Chicago, Illinois 60637, United States.
Howard Hughes Medical Institute, The University of Chicago, Chicago, Illinois 60637, United States.

Boxuan Simen Zhao (BS)

Department of Genetics, Stanford University, Stanford, California 94305, United States.

Pradnya Narkhede (P)

Department of Chemistry, University of Cambridge, Cambridge CB2 0SP, U.K.

Yawei Gao (Y)

Clinical and Translational Research Center of Shanghai First Maternity and Infant Hospital, Shanghai Key Laboratory of Signaling and Disease Research, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China.

Jun Liu (J)

Department of Chemistry, Department of Biochemistry and Molecular Biology, and Institute for Biophysical Dynamics, The University of Chicago, Chicago, Illinois 60637, United States.
Howard Hughes Medical Institute, The University of Chicago, Chicago, Illinois 60637, United States.

Xiaoyang Dou (X)

Department of Chemistry, Department of Biochemistry and Molecular Biology, and Institute for Biophysical Dynamics, The University of Chicago, Chicago, Illinois 60637, United States.
Howard Hughes Medical Institute, The University of Chicago, Chicago, Illinois 60637, United States.

Qing Dai (Q)

Department of Chemistry, Department of Biochemistry and Molecular Biology, and Institute for Biophysical Dynamics, The University of Chicago, Chicago, Illinois 60637, United States.
Howard Hughes Medical Institute, The University of Chicago, Chicago, Illinois 60637, United States.

Li-Sheng Zhang (LS)

Department of Chemistry, Department of Biochemistry and Molecular Biology, and Institute for Biophysical Dynamics, The University of Chicago, Chicago, Illinois 60637, United States.
Howard Hughes Medical Institute, The University of Chicago, Chicago, Illinois 60637, United States.

Chuan He (C)

Department of Chemistry, Department of Biochemistry and Molecular Biology, and Institute for Biophysical Dynamics, The University of Chicago, Chicago, Illinois 60637, United States.
Howard Hughes Medical Institute, The University of Chicago, Chicago, Illinois 60637, United States.
ORCID: 0000-0003-4319-7424

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Classifications MeSH

questionsmedicales.fr › Composés hétérocycliques › Composés hétéromonocycliques › Pyrimidines › Pyrimidinones › Cytosine › 5-Méthyl-cytosine › DNA 5-Methylcytosine-Specific Amplification...
questionsmedicales.fr › Eucaryotes › Animaux › DNA 5-Methylcytosine-Specific Amplification...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ADN › DNA 5-Methylcytosine-Specific Amplification...
questionsmedicales.fr › Phénomènes génétiques › Méthylation de l'ADN › DNA 5-Methylcytosine-Specific Amplification...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › DNA 5-Methylcytosine-Specific Amplification...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › DNA 5-Methylcytosine-Specific Amplification...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques d'amplification d'acides nucléiques › DNA 5-Methylcytosine-Specific Amplification...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Analyse de séquence › Analyse de séquence d'ADN › DNA 5-Methylcytosine-Specific Amplification...
questionsmedicales.fr › Produits chimiques inorganiques › Sulfites › DNA 5-Methylcytosine-Specific Amplification...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Analyse de séquence › Analyse de séquence d'ADN › Séquençage du génome entier › DNA 5-Methylcytosine-Specific Amplification...