New opportunities and insights into Papaver self-incompatibility by imaging engineered Arabidopsis pollen.
Actin
actin-binding proteins (ABPs)
calcium
endocytosis
fluorescent probes
live-cell imaging
pH
pollen tube growth
programmed cell death (PCD)
self-incompatibility (SI)
Journal
Journal of experimental botany
ISSN: 1460-2431
Titre abrégé: J Exp Bot
Pays: England
ID NLM: 9882906
Informations de publication
Date de publication:
23 04 2020
23 04 2020
Historique:
received:
05
11
2019
accepted:
25
02
2020
pubmed:
27
2
2020
medline:
11
5
2021
entrez:
27
2
2020
Statut:
ppublish
Résumé
Pollen tube growth is essential for plant reproduction. Their rapid extension using polarized tip growth provides an exciting system for studying this specialized type of growth. Self-incompatibility (SI) is a genetically controlled mechanism to prevent self-fertilization. Mechanistically, one of the best-studied SI systems is that of Papaver rhoeas (poppy). This utilizes two S-determinants: stigma-expressed PrsS and pollen-expressed PrpS. Interaction of cognate PrpS-PrsS triggers a signalling network, causing rapid growth arrest and programmed cell death (PCD) in incompatible pollen. We previously demonstrated that transgenic Arabidopsis thaliana pollen expressing PrpS-green fluorescent protein (GFP) can respond to Papaver PrsS with remarkably similar responses to those observed in incompatible Papaver pollen. Here we describe recent advances using these transgenic plants combined with genetically encoded fluorescent probes to monitor SI-induced cellular alterations, including cytosolic calcium, pH, the actin cytoskeleton, clathrin-mediated endocytosis (CME), and the vacuole. This approach has allowed us to study the SI response in depth, using multiparameter live-cell imaging approaches that were not possible in Papaver. This lays the foundations for new opportunities to elucidate key mechanisms involved in SI. Here we establish that CME is disrupted in self-incompatible pollen. Moreover, we reveal new detailed information about F-actin remodelling in pollen tubes after SI.
Identifiants
pubmed: 32100005
pii: 5758054
doi: 10.1093/jxb/eraa092
pmc: PMC7178406
doi:
Substances chimiques
Plant Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2451-2463Subventions
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/P005489/1
Pays : United Kingdom
Informations de copyright
© The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology.
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