Dense Transposon Integration Reveals Essential Cleavage and Polyadenylation Factors Promote Heterochromatin Formation.
Cell Nucleus
/ metabolism
Centromere
/ metabolism
DNA Transposable Elements
/ genetics
Exosomes
/ metabolism
Gene Expression Regulation, Fungal
Heterochromatin
/ metabolism
Meiosis
/ genetics
RNA Interference
RNA Processing, Post-Transcriptional
/ genetics
RNA, Fungal
/ genetics
RNA, Messenger
/ genetics
Schizosaccharomyces
/ genetics
Schizosaccharomyces pombe Proteins
/ metabolism
mRNA Cleavage and Polyadenylation Factors
/ metabolism
Iss1
Mmi1
RNA elimination
Rrp6
Schizosaccharomyces pombe
Tn-seq
heterochromatin
mei4
polyadenylation
ssm4
Journal
Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691
Informations de publication
Date de publication:
25 02 2020
25 02 2020
Historique:
received:
18
04
2019
revised:
18
10
2019
accepted:
27
01
2020
entrez:
27
2
2020
pubmed:
27
2
2020
medline:
18
3
2021
Statut:
ppublish
Résumé
Heterochromatin functions as a scaffold for factors responsible for gene silencing and chromosome segregation. Heterochromatin can be assembled by multiple pathways, including RNAi and RNA surveillance. We identified factors that form heterochromatin using dense profiles of transposable element integration in Schizosaccharomyces pombe. The candidates include a large number of essential proteins such as four canonical mRNA cleavage and polyadenylation factors. We find that Iss1, a subunit of the poly(A) polymerase module, plays a role in forming heterochromatin in centromere repeats that is independent of RNAi. Genome-wide maps reveal that Iss1 accumulates at genes regulated by RNA surveillance. Iss1 interacts with RNA surveillance factors Mmi1 and Rrp6, and importantly, Iss1 contributes to RNA elimination that forms heterochromatin at meiosis genes. Our profile of transposable element integration supports the model that a network of mRNA cleavage and polyadenylation factors coordinates RNA surveillance, including the mechanism that forms heterochromatin at meiotic genes.
Identifiants
pubmed: 32101745
pii: S2211-1247(20)30128-5
doi: 10.1016/j.celrep.2020.01.094
pmc: PMC9497450
mid: NIHMS1719285
pii:
doi:
Substances chimiques
DNA Transposable Elements
0
Heterochromatin
0
RNA, Fungal
0
RNA, Messenger
0
Schizosaccharomyces pombe Proteins
0
mRNA Cleavage and Polyadenylation Factors
0
Types de publication
Journal Article
Research Support, N.I.H., Intramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
2686-2698.e8Subventions
Organisme : Intramural NIH HHS
ID : ZIA HD001009
Pays : United States
Organisme : Intramural NIH HHS
ID : ZIA HD008957
Pays : United States
Informations de copyright
Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Interests The authors declare no competing interests.
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