Organismal benefits of transcription speed control at gene boundaries.
NET-seq
speed
splicing
stalling
transcription
Journal
EMBO reports
ISSN: 1469-3178
Titre abrégé: EMBO Rep
Pays: England
ID NLM: 100963049
Informations de publication
Date de publication:
03 04 2020
03 04 2020
Historique:
received:
19
09
2019
revised:
24
01
2020
accepted:
30
01
2020
pubmed:
28
2
2020
medline:
28
4
2021
entrez:
28
2
2020
Statut:
ppublish
Résumé
RNA polymerase II (RNAPII) transcription is crucial for gene expression. RNAPII density peaks at gene boundaries, associating these key regions for gene expression control with limited RNAPII movement. The connections between RNAPII transcription speed and gene regulation in multicellular organisms are poorly understood. Here, we directly modulate RNAPII transcription speed by point mutations in the second largest subunit of RNAPII in Arabidopsis thaliana. A RNAPII mutation predicted to decelerate transcription is inviable, while accelerating RNAPII transcription confers phenotypes resembling auto-immunity. Nascent transcription profiling revealed that RNAPII complexes with accelerated transcription clear stalling sites at both gene ends, resulting in read-through transcription. The accelerated transcription mutant NRPB2-Y732F exhibits increased association with 5' splice site (5'SS) intermediates and enhanced splicing efficiency. Our findings highlight potential advantages of RNAPII stalling through local reduction in transcription speed to optimize gene expression for the development of multicellular organisms.
Identifiants
pubmed: 32103605
doi: 10.15252/embr.201949315
pmc: PMC7132196
doi:
Substances chimiques
Arabidopsis Proteins
0
RNA Polymerase II
EC 2.7.7.-
Banques de données
GEO
['GSE133143']
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e49315Subventions
Organisme : EC | Horizon 2020 Framework Programme (H2020)
ID : MSCA-IF 703085
Pays : International
Organisme : EC | Horizon 2020 Framework Programme (H2020)
ID : StG2017-757411
Pays : International
Organisme : NIGMS NIH HHS
ID : R01 GM097260
Pays : United States
Organisme : Novo Nordisk Fonden (NNF)
ID : NNF15OC0014202
Pays : International
Organisme : Copenhagen Plant Science Centre
Pays : International
Informations de copyright
© 2020 The Authors. Published under the terms of the CC BY NC ND 4.0 license.
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