Epidemiology of colistin-resistant, carbapenemase-producing Enterobacteriaceae and Acinetobacter baumannii in Croatia.
Acinetobacter Infections
/ drug therapy
Acinetobacter baumannii
/ drug effects
Anti-Bacterial Agents
/ therapeutic use
Bacterial Proteins
/ drug effects
Colistin
/ therapeutic use
Croatia
Cross Infection
/ drug therapy
Drug Resistance, Multiple, Bacterial
/ genetics
Enterobacteriaceae
/ drug effects
Enterobacteriaceae Infections
/ drug therapy
Humans
Microbial Sensitivity Tests
/ methods
Phylogeny
Whole Genome Sequencing
/ methods
beta-Lactamases
/ drug effects
A. baumannii
Carbapenemase
Colistin resistance
Enterobacteriaceae
Journal
Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases
ISSN: 1567-7257
Titre abrégé: Infect Genet Evol
Pays: Netherlands
ID NLM: 101084138
Informations de publication
Date de publication:
07 2020
07 2020
Historique:
received:
11
12
2019
revised:
19
02
2020
accepted:
21
02
2020
pubmed:
28
2
2020
medline:
29
6
2021
entrez:
28
2
2020
Statut:
ppublish
Résumé
Colistin is a last-resort antibiotic for the treatment of infections caused by multidrug and carbapenem-resistant Gram-negative bacteria. Colistin resistance has been emerging and multiple outbreaks have been reported in Europe and elsewhere. It has been most frequently reported in carbapenem-resistant K. pneumoniae. In this study, 24 multidrug and colistin-resistant clinical isolates (14 K. pneumoniae, one E. aerogenes, one E. cloacae, and eight A. baumannii) were collected from four hospitals in Croatia from 2013 to 2018, in order to analyse the molecular epidemiology and mechanisms of antibiotic resistance. β-lactamase and carbapenemase genes were detected by PCR. Genotyping was done on selected isolates by rep-PCR. Whole genome sequencing (WGS) was performed to discover possible molecular mechanisms for the observed colistin resistance. All isolates, except two K. pneumoniae isolates, were extensively drug resistant. Ten out of 16 (63%) K. pneumoniae isolates possessed bla
Identifiants
pubmed: 32105865
pii: S1567-1348(20)30094-0
doi: 10.1016/j.meegid.2020.104263
pii:
doi:
Substances chimiques
Anti-Bacterial Agents
0
Bacterial Proteins
0
beta-Lactamases
EC 3.5.2.6
carbapenemase
EC 3.5.2.6
Colistin
Z67X93HJG1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
104263Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.