ESCRT-III/Vps4 Controls Heterochromatin-Nuclear Envelope Attachments.

Chromatin / metabolism Endosomal Sorting Complexes Required for Transport / metabolism Heterochromatin / metabolism Membrane Proteins / metabolism Mitosis / physiology Nuclear Envelope / metabolism Nuclear Proteins / metabolism Schizosaccharomyces / metabolism Schizosaccharomyces pombe Proteins / metabolism
ESCRT-III Lem2 Vps4 fission yeast heterochromatin nuclear envelope

Journal

Developmental cell
ISSN: 1878-1551
Titre abrégé: Dev Cell
Pays: United States
ID NLM: 101120028

Informations de publication

Date de publication:
06 04 2020
Historique:
received: 24 04 2019
revised: 05 12 2019
accepted: 27 01 2020
pubmed: 29 2 2020
medline: 24 10 2020
entrez: 29 2 2020
Statut: ppublish

Résumé

Eukaryotic genomes are organized within the nucleus through interactions with inner nuclear membrane (INM) proteins. How chromatin tethering to the INM is controlled in interphase and how this process contributes to subsequent mitotic nuclear envelope (NE) remodeling remains unclear. We have probed these fundamental questions using the fission yeast Schizosaccharomyces japonicus, which breaks and reforms the NE during mitosis. We show that attachments between heterochromatin and the transmembrane Lem2-Nur1 complex at the INM are remodeled in interphase by the ESCRT-III/Vps4 machinery. Failure of ESCRT-III/Vps4 to release Lem2-Nur1 from heterochromatin leads to persistent association of chromosomes with the INM throughout mitosis. At mitotic exit, such trapping of Lem2-Nur1 on heterochromatin prevents it from re-establishing nucleocytoplasmic compartmentalization. Our work identifies the Lem2-Nur1 complex as a substrate for the nuclear ESCRT machinery and explains how the dynamic tethering of chromosomes to the INM is linked to the establishment of nuclear compartmentalization.

Identifiants

DOI: 10.1016/j.devcel.2020.01.028 PMID: 32109380 PMC: PMC7139201
pubmed: 32109380
pii: S1534-5807(20)30060-5
doi: 10.1016/j.devcel.2020.01.028
pmc: PMC7139201
mid: EMS85884
pii:
doi:

Substances chimiques

Chromatin 0
Endosomal Sorting Complexes Required for Transport 0
Heterochromatin 0
Membrane Proteins 0
Nuclear Proteins 0
Schizosaccharomyces pombe Proteins 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

27-41.e6

Subventions

Organisme : Wellcome Trust
ID : 103741
Pays : United Kingdom
Organisme : Cancer Research UK
ID : FC001002
Pays : United Kingdom
Organisme : Medical Research Council
ID : FC001002
Pays : United Kingdom
Organisme : Wellcome Trust
ID : FC001002
Pays : United Kingdom

Commentaires et corrections

Type : CommentIn

Informations de copyright

Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of Interests The authors declare no competing interests.

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Auteurs

Gerard H Pieper (GH)

The Francis Crick Institute, 1 Midland Road, London NW1 1AT, UK; Randall Centre for Cell and Molecular Biophysics, School of Basic and Medical Biosciences, King's College London, London SE1 1UL, UK.

Simon Sprenger (S)

Institute for Cell Biology, Biocenter, Medical University of Innsbruck, Innrain 80/82, A-6020, Innsbruck, Austria.

David Teis (D)

Institute for Cell Biology, Biocenter, Medical University of Innsbruck, Innrain 80/82, A-6020, Innsbruck, Austria. Electronic address: david.teis@i-med.ac.at.

Snezhana Oliferenko (S)

The Francis Crick Institute, 1 Midland Road, London NW1 1AT, UK; Randall Centre for Cell and Molecular Biophysics, School of Basic and Medical Biosciences, King's College London, London SE1 1UL, UK. Electronic address: snezhka.oliferenko@crick.ac.uk.

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Classifications MeSH

questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Structures de chromosome › Chromatine › ESCRT-III/Vps4 Controls...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Protéines du transport vésiculaire › Complexes de tri endosomique requis pour le transport › ESCRT-III/Vps4 Controls...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Structures de chromosome › Chromatine › Hétérochromatine › ESCRT-III/Vps4 Controls...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › ESCRT-III/Vps4 Controls...
questionsmedicales.fr › Phénomènes génétiques › Division du noyau cellulaire › Mitose › ESCRT-III/Vps4 Controls...
questionsmedicales.fr › Cellules › Structures cellulaires › Fractions subcellulaires › Membranes intracellulaires › Enveloppe nucléaire › ESCRT-III/Vps4 Controls...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines nucléaires › ESCRT-III/Vps4 Controls...
questionsmedicales.fr › Eucaryotes › Champignons › Levures › Schizosaccharomyces › ESCRT-III/Vps4 Controls...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines fongiques › Protéines de Schizosaccharomyces pombe › ESCRT-III/Vps4 Controls...