Stem rust resistance in wheat is suppressed by a subunit of the mediator complex.

Basidiomycota / pathogenicity Chromosome Mapping Chromosomes, Plant / genetics Disease Resistance / genetics Gene Duplication Gene Expression Gene Expression Regulation, Plant Genes, Plant / genetics Mediator Complex / genetics Mutation Phenotype Plant Diseases / genetics Plant Immunity / genetics Poaceae / classification Triticum / genetics

Journal

Nature communications

ISSN: 2041-1723

Titre abrégé: Nat Commun

Pays: England

ID NLM: 101528555

Informations de publication

Date de publication:
28 02 2020

Historique:

received: 31 12 2019

accepted: 11 02 2020

entrez: 1 3 2020

pubmed: 1 3 2020

medline: 27 5 2020

Statut: epublish

Résumé

Stem rust is an important disease of wheat that can be controlled using resistance genes. The gene SuSr-D1 identified in cultivar 'Canthatch' suppresses stem rust resistance. SuSr-D1 mutants are resistant to several races of stem rust that are virulent on wild-type plants. Here we identify SuSr-D1 by sequencing flow-sorted chromosomes, mutagenesis, and map-based cloning. The gene encodes Med15, a subunit of the Mediator Complex, a conserved protein complex in eukaryotes that regulates expression of protein-coding genes. Nonsense mutations in Med15b.D result in expression of stem rust resistance. Time-course RNAseq analysis show a significant reduction or complete loss of differential gene expression at 24 h post inoculation in med15b.D mutants, suggesting that transcriptional reprogramming at this time point is not required for immunity to stem rust. Suppression is a common phenomenon and this study provides novel insight into suppression of rust resistance in wheat.

Identifiants

DOI: 10.1038/s41467-020-14937-2 PMID: 32111840 PMC: PMC7048732

pubmed: 32111840

doi: 10.1038/s41467-020-14937-2

pii: 10.1038/s41467-020-14937-2

pmc: PMC7048732

doi:

Substances chimiques

Mediator Complex 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Sous-ensembles de citation

Pagination

1123

Subventions

Organisme : National Science Foundation (NSF)

ID : IOS-0965429

Pays : International

Organisme : Biotechnology and Biological Sciences Research Council

ID : BB/P012574/1

Pays : United Kingdom

Organisme : Biotechnology and Biological Sciences Research Council

ID : BB/P016855/1

Pays : United Kingdom

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