Targeted gene panel use in 2249 neuromuscular patients: the Australasian referral center experience.


Journal

Annals of clinical and translational neurology
ISSN: 2328-9503
Titre abrégé: Ann Clin Transl Neurol
Pays: United States
ID NLM: 101623278

Informations de publication

Date de publication:
03 2020
Historique:
received: 16 12 2019
revised: 28 01 2020
accepted: 06 02 2020
pubmed: 11 3 2020
medline: 13 4 2021
entrez: 11 3 2020
Statut: ppublish

Résumé

To develop, test, and iterate a comprehensive neuromuscular targeted gene panel in a national referral center. We designed two iterations of a comprehensive targeted gene panel for neuromuscular disorders. Version 1 included 336 genes, which was increased to 464 genes in Version 2. Both panels used TargetSeq Six hundred and sixty-five patients received a genetic diagnosis (30%). Diagnosed patients were significantly younger that undiagnosed patients (26.4 and 32.5 years, respectively; P = 4.6326E-9). The diagnostic success varied markedly between disease categories. Pathogenic variants in 10 genes explained 38% of the disease burden. Unexpected phenotypic expansions were discovered in multiple cases. Triage of unsolved cases for research exome testing led to the discovery of six new disease genes. A comprehensive targeted diagnostic panel was an effective method for neuromuscular disease diagnosis within the context of an Australasian referral center. Use of smaller disease-specific panels would have precluded diagnosis in many patients and increased cost. Analysis through a centralized laboratory facilitated detection of recurrent, but under-recognized pathogenic variants.

Identifiants

pubmed: 32153140
doi: 10.1002/acn3.51002
pmc: PMC7086001
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

353-362

Informations de copyright

© 2020 The Authors. Annals of Clinical and Translational Neurology published by Wiley Periodicals, Inc on behalf of American Neurological Association.

Références

J Neuromuscul Dis. 2018;5(3):307-314
pubmed: 30040739
Am J Hum Genet. 2015 Jun 4;96(6):955-61
pubmed: 26004201
Am J Hum Genet. 2016 Sep 1;99(3):666-673
pubmed: 27523598
Brain. 2012 Oct;135(Pt 10):2994-3004
pubmed: 22964162
Neuromuscul Disord. 2017 Jul;27(7):607-615
pubmed: 28554554
Nat Genet. 1993 Jul;4(3):314-20
pubmed: 8358442
Ann Neurol. 2018 Jun;83(6):1105-1124
pubmed: 29691892
Brain. 2011 Jan;134(Pt 1):171-182
pubmed: 21186264
Nat Rev Genet. 2011 May;12(5):363-76
pubmed: 21358748
Am J Hum Genet. 2018 Mar 1;102(3):505-514
pubmed: 29499166
Clin Genet. 2018 Jan;93(1):78-83
pubmed: 28378410
Genet Med. 2018 Sep;20(9):1045-1053
pubmed: 29095814
Genome Res. 2009 Sep;19(9):1527-41
pubmed: 19546169
J Neurol. 2013 May;260(5):1286-94
pubmed: 23269439
Mol Genet Genomic Med. 2014 Nov;2(6):522-9
pubmed: 25614874
Neuromuscul Disord. 2016 Aug;26(8):504-10
pubmed: 27312022
Neuromuscul Disord. 2011 Dec;21(12):833-61
pubmed: 22250299
Neurol Genet. 2018 Feb 01;4(1):e212
pubmed: 29417091
JAMA Neurol. 2018 Oct 1;75(10):1234-1245
pubmed: 29913018
J Med Genet. 1997 Jun;34(6):470-5
pubmed: 9192266
Neurol Neuroimmunol Neuroinflamm. 2018 Dec 12;6(1):e523
pubmed: 30588482
Methods Mol Biol. 2011;688:97-126
pubmed: 20938835
Mol Cells. 2016 May 31;39(5):382-8
pubmed: 27025386
Nucleic Acids Res. 2010 Sep;38(16):e164
pubmed: 20601685
Orphanet J Rare Dis. 2013 Mar 15;8:41
pubmed: 23497566
NPJ Genom Med. 2017;2:
pubmed: 29152331
BMC Med Genet. 2015 Sep 21;16:84
pubmed: 26392352
Eur J Neurol. 2018 Jun;25(6):841-847
pubmed: 29498452
Hum Mol Genet. 2018 Dec 15;27(24):4263-4272
pubmed: 30215711
Genet Med. 2015 May;17(5):405-24
pubmed: 25741868
Clin Genet. 2018 Feb;93(2):301-309
pubmed: 28708278
Brain. 2016 Nov 1;139(11):2844-2854
pubmed: 27604308
Neuromuscul Disord. 2013 Dec;23(12):1081-111
pubmed: 24446552
Genet Med. 2018 Dec;20(12):1554-1563
pubmed: 29543227
Nat Commun. 2019 Mar 27;10(1):1396
pubmed: 30918256
Genet Med. 2019 Jan;21(1):173-180
pubmed: 29765138
Neuromuscul Disord. 2018 Dec;28(12):1031-1063
pubmed: 30472062
Clin Genet. 2011 Oct;80(4):334-45
pubmed: 21291453
Neuromuscul Disord. 2016 Nov;26(11):744-748
pubmed: 27751653
Brain. 2015 Apr;138(Pt 4):836-44
pubmed: 25681410
J Neurol. 2013 Aug;260(8):2084-93
pubmed: 23670307
Genome Biol. 2015 Oct 13;16:226
pubmed: 26463453
Neuromuscul Disord. 2017 Nov;27(11):997-1008
pubmed: 28967462
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7
pubmed: 271968
Hum Mutat. 2018 Sep;39(9):1284-1298
pubmed: 29858556
Brain. 2016 Mar;139(Pt 3):674-91
pubmed: 26700687
Neurol Genet. 2015 Aug 13;1(2):e14
pubmed: 27066551
J Med Genet. 2004 Apr;41(4):e50
pubmed: 15060126
F1000Res. 2018 Dec 11;7:
pubmed: 30631434
Neurology. 2003 Jun 10;60(11):1805-10
pubmed: 12796535

Auteurs

Sarah J Beecroft (SJ)

Centre for Medical Research, University of Western Australia, Harry Perkins Institute of Medical Research, QEII Medical Centre, Nedlands, Western Australia, Australia.

Kyle S Yau (KS)

Centre for Medical Research, University of Western Australia, Harry Perkins Institute of Medical Research, QEII Medical Centre, Nedlands, Western Australia, Australia.

Richard J N Allcock (RJN)

School of Biomedical Sciences, University of Western Australia, Perth, Western Australia, Australia.

Kym Mina (K)

Department of Diagnostic Genomics, Department of Health, PathWest Laboratory Medicine, QEII Medical Centre, Nedlands, Western Australia, Australia.

Rebecca Gooding (R)

Department of Diagnostic Genomics, Department of Health, PathWest Laboratory Medicine, QEII Medical Centre, Nedlands, Western Australia, Australia.

Fathimath Faiz (F)

Department of Diagnostic Genomics, Department of Health, PathWest Laboratory Medicine, QEII Medical Centre, Nedlands, Western Australia, Australia.

Vanessa J Atkinson (VJ)

School of Biomedical Sciences, University of Western Australia, Perth, Western Australia, Australia.
Department of Diagnostic Genomics, Department of Health, PathWest Laboratory Medicine, QEII Medical Centre, Nedlands, Western Australia, Australia.

Cheryl Wise (C)

Department of Diagnostic Genomics, Department of Health, PathWest Laboratory Medicine, QEII Medical Centre, Nedlands, Western Australia, Australia.

Padma Sivadorai (P)

Department of Diagnostic Genomics, Department of Health, PathWest Laboratory Medicine, QEII Medical Centre, Nedlands, Western Australia, Australia.

Daniel Trajanoski (D)

Department of Diagnostic Genomics, Department of Health, PathWest Laboratory Medicine, QEII Medical Centre, Nedlands, Western Australia, Australia.

Nina Kresoje (N)

School of Biomedical Sciences, University of Western Australia, Perth, Western Australia, Australia.

Royston Ong (R)

Centre for Medical Research, University of Western Australia, Harry Perkins Institute of Medical Research, QEII Medical Centre, Nedlands, Western Australia, Australia.

Rachael M Duff (RM)

Centre for Medical Research, University of Western Australia, Harry Perkins Institute of Medical Research, QEII Medical Centre, Nedlands, Western Australia, Australia.

Macarena Cabrera-Serrano (M)

Department of Neurology, Hospital Universitario Virgen del Rocio, Instituto de Biomedicina de Sevilla, CSIC, Universidad de Sevilla, Sevilla, Spain.

Kristen J Nowak (KJ)

Centre for Medical Research, University of Western Australia, Harry Perkins Institute of Medical Research, QEII Medical Centre, Nedlands, Western Australia, Australia.
Public and Aboriginal Health Division, Department of Health, Office of Population Health Genomics, Perth, Western Australia, Australia.

Nicholas Pachter (N)

Genetic Services of Western Australia, Department of Health, Government of Western Australia, Perth, Western Australia, Australia.
School of Paediatrics and Child Health, University of Western Australia, Perth, Western Australia, Australia.

Gianina Ravenscroft (G)

Centre for Medical Research, University of Western Australia, Harry Perkins Institute of Medical Research, QEII Medical Centre, Nedlands, Western Australia, Australia.

Phillipa J Lamont (PJ)

Neurogenetic Unit, Royal Perth Hospital, Perth, Western Australia, Australia.

Mark R Davis (MR)

Department of Diagnostic Genomics, Department of Health, PathWest Laboratory Medicine, QEII Medical Centre, Nedlands, Western Australia, Australia.

Nigel G Laing (NG)

Centre for Medical Research, University of Western Australia, Harry Perkins Institute of Medical Research, QEII Medical Centre, Nedlands, Western Australia, Australia.
Department of Diagnostic Genomics, Department of Health, PathWest Laboratory Medicine, QEII Medical Centre, Nedlands, Western Australia, Australia.

Articles similaires

Genome, Chloroplast Phylogeny Genetic Markers Base Composition High-Throughput Nucleotide Sequencing

[Redispensing of expensive oral anticancer medicines: a practical application].

Lisanne N van Merendonk, Kübra Akgöl, Bastiaan Nuijen
1.00
Humans Antineoplastic Agents Administration, Oral Drug Costs Counterfeit Drugs

Smoking Cessation and Incident Cardiovascular Disease.

Jun Hwan Cho, Seung Yong Shin, Hoseob Kim et al.
1.00
Humans Male Smoking Cessation Cardiovascular Diseases Female
Humans United States Aged Cross-Sectional Studies Medicare Part C

Classifications MeSH