Dynorphin and GABAA Receptor Signaling Contribute to Progesterone's Inhibition of the LH Surge in Female Mice.


Journal

Endocrinology
ISSN: 1945-7170
Titre abrégé: Endocrinology
Pays: United States
ID NLM: 0375040

Informations de publication

Date de publication:
01 05 2020
Historique:
received: 07 09 2019
accepted: 28 02 2020
pubmed: 18 3 2020
medline: 5 1 2021
entrez: 18 3 2020
Statut: ppublish

Résumé

Progesterone can block estrogen-induced luteinising hormone (LH) surge secretion and can be used clinically to prevent premature LH surges. The blocking effect of progesterone on the LH surge is mediated through its receptor in the anteroventral periventricular nucleus (AVPV) of the hypothalamus. However, the underlying mechanisms are unclear. The preovulatory LH surge induced by estrogen is preceded by a significant reduction in hypothalamic dynorphin and gamma-aminobutyric acid (GABA) release. To test the detailed roles of dynorphin and GABA in an LH surge blockade by progesterone, ovariectomized and 17β-estradiol capsule-implanted (OVX/E2) mice received simultaneous injections of estradiol benzoate (EB) and progesterone (P) or vehicle for 2 consecutive days. The LH level was monitored from 2:30 pm to 8:30 pm at 30-minute intervals. Progesterone coadministration resulted in the LH surge blockade. A continuous microinfusion of the dynorphin receptor antagonist nor-BNI or GABAA receptor antagonist bicuculline into the AVPV from 3:00 pm to 7:00 pm reversed the progesterone-mediated blockade of the LH surge in 7 of 9 and 6 of 10 mice, respectively. In addition, these LH surges started much earlier than the surge induced by estrogen alone. However, 5 of 7 progesterone-treated mice did not show LH surge secretion after microinfusion with the GABAB receptor antagonist CGP-35348. Additionally, peripheral administration of kisspeptin-54 promotes LH surge-like release in progesterone treated mice. These results demonstrated that the progesterone-mediated suppression of the LH surge is mediated by an increase in dynorphin and GABAA receptor signaling acting though kisspeptin neurons in the AVPV of the hypothalamus in female mice.

Identifiants

pubmed: 32181477
pii: 5808894
doi: 10.1210/endocr/bqaa036
pmc: PMC7153819
pii:
doi:

Substances chimiques

GABA Antagonists 0
Kisspeptins 0
Organophosphorus Compounds 0
Receptors, GABA-A 0
norbinaltorphimine 36OOQ86QM1
Progesterone 4G7DS2Q64Y
Estradiol 4TI98Z838E
Naltrexone 5S6W795CQM
Dynorphins 74913-18-1
CGP 35348 87TI61875H
Luteinizing Hormone 9002-67-9
Bicuculline Y37615DVKC

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Commentaires et corrections

Type : ErratumIn

Informations de copyright

© Endocrine Society 2020.

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Auteurs

Yali Liu (Y)

Department of Assisted Reproduction, Shanghai Ninth People's Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Huangpu District, Shanghai, China.
Department of Women and Children's Health, Faculty of Life Sciences and Medicine, King's College London, Guy's Campus, UK.
Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine, Pudong New Area, Shanghai, China.

Xiaofeng Li (X)

Department of Women and Children's Health, Faculty of Life Sciences and Medicine, King's College London, Guy's Campus, UK.

Deyana Ivanova (D)

Department of Women and Children's Health, Faculty of Life Sciences and Medicine, King's College London, Guy's Campus, UK.

Geffen Lass (G)

Department of Women and Children's Health, Faculty of Life Sciences and Medicine, King's College London, Guy's Campus, UK.

Wen He (W)

Department of Assisted Reproduction, Shanghai Ninth People's Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Huangpu District, Shanghai, China.

Qiuju Chen (Q)

Department of Assisted Reproduction, Shanghai Ninth People's Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Huangpu District, Shanghai, China.

Sha Yu (S)

Department of Assisted Reproduction, Shanghai Ninth People's Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Huangpu District, Shanghai, China.

Yun Wang (Y)

Department of Assisted Reproduction, Shanghai Ninth People's Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Huangpu District, Shanghai, China.

Hui Long (H)

Department of Assisted Reproduction, Shanghai Ninth People's Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Huangpu District, Shanghai, China.

Li Wang (L)

Department of Assisted Reproduction, Shanghai Ninth People's Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Huangpu District, Shanghai, China.

Qifeng Lyu (Q)

Department of Assisted Reproduction, Shanghai Ninth People's Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Huangpu District, Shanghai, China.

Yanping Kuang (Y)

Department of Assisted Reproduction, Shanghai Ninth People's Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Huangpu District, Shanghai, China.

Kevin T O'Byrne (KT)

Department of Women and Children's Health, Faculty of Life Sciences and Medicine, King's College London, Guy's Campus, UK.

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Classifications MeSH