Site-Directed Antibody Immobilization by Resorc[4]arene-Based Immunosensors.

Antibodies / chemistry Antibodies, Immobilized / chemistry Binding Sites Gold / chemistry Surface Plasmon Resonance / methods
immunosensor macrocycles resorc[4]arene site directed immobilization surface plasmon resonance

Journal

Chemistry (Weinheim an der Bergstrasse, Germany)
ISSN: 1521-3765
Titre abrégé: Chemistry
Pays: Germany
ID NLM: 9513783

Informations de publication

Date de publication:
08 Jul 2020
Historique:
received: 24 02 2020
revised: 24 03 2020
pubmed: 3 4 2020
medline: 21 10 2020
entrez: 3 4 2020
Statut: ppublish

Résumé

One of the main problems in the development of immunosensors is to overcome the complexity of binding antibodies to the sensor surface. Most immobilizing methods lead to a random orientation of antibodies with a lower binding site density and immunoaffinity. In order to control the orientation of antibody immobilization, several resorc[4]arene derivatives were designed and synthesized. After the spectroscopic characterization of resorc[4]arene self-assembled monolayers (SAMs) onto gold films, the surface coverage and the orientation of insulin antibody (Ab-Ins) were assessed by a surface plasmon resonance (SPR) technique and compared with a random immobilization method. Experimental results combined with theoretical studies confirmed the dipole-dipole interaction as an important factor in antibody orientation and demonstrated the importance of the upper rim functionalization of resorcarenes. Accordingly, resorcarene 5 showed a major binding force towards Ab-Ins thanks to the H-bond interactions with the amine protein groups. Based on these findings, the resorcarene-based immunosensor is a powerful system with improved sensitivity providing new insight into sensor development.

Identifiants

DOI: 10.1002/chem.202000989 PMID: 32240571
pubmed: 32240571
doi: 10.1002/chem.202000989
doi:

Substances chimiques

Antibodies 0
Antibodies, Immobilized 0
Gold 7440-57-5

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

8400-8406

Subventions

Organisme : PON (Piano Operativo Nazionale)
ID : ARS01_00432 PROGEMA
Organisme : Ministero dell'Istruzione, dell'Università e della Ricerca
ID : PRIN 2017 - "Tar-geting Hedgehog pathway: Virtual screening identification and sustainable synthesis of novel Smo and Gli inhibitors and their pharmacological drug delivery strategies for im-proved therapeutic effects in tumors"
Organisme : Ministero dell'Istruzione, dell'Università e della Ricerca
ID : Dipartimenti di Eccellenza - L. 232/2016
Organisme : Sapienza Università di Roma
ID : project RM11816436113D8A
Organisme : Regione Lazio
ID : NanoBioPOCT25-OHD

Informations de copyright

© 2020 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

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Auteurs

Deborah Quaglio (D)

Department of Chemistry and Technology of Drugs, Department of Excellence 2018-2022, Sapienza University of Rome, P.le Aldo Moro 5, 00185, Rome, Italy.

Laura Mangiardi (L)

Department of Chemistry and Technology of Drugs, Department of Excellence 2018-2022, Sapienza University of Rome, P.le Aldo Moro 5, 00185, Rome, Italy.
Center For Life Nano Science@Sapienza, Istituto Italiano di Tecnologia, Viale Regina Elena 291, 00161, Rome, Italy.

Giulia Venditti (G)

Department of Chemistry and Technology of Drugs, Department of Excellence 2018-2022, Sapienza University of Rome, P.le Aldo Moro 5, 00185, Rome, Italy.

Cristina Del Plato (C)

Department of Chemistry and Technology of Drugs, Department of Excellence 2018-2022, Sapienza University of Rome, P.le Aldo Moro 5, 00185, Rome, Italy.
Center For Life Nano Science@Sapienza, Istituto Italiano di Tecnologia, Viale Regina Elena 291, 00161, Rome, Italy.

Francesca Polli (F)

Department of Chemistry and Technology of Drugs, Department of Excellence 2018-2022, Sapienza University of Rome, P.le Aldo Moro 5, 00185, Rome, Italy.

Francesca Ghirga (F)

Center For Life Nano Science@Sapienza, Istituto Italiano di Tecnologia, Viale Regina Elena 291, 00161, Rome, Italy.

Gabriele Favero (G)

Department of Chemistry and Technology of Drugs, Department of Excellence 2018-2022, Sapienza University of Rome, P.le Aldo Moro 5, 00185, Rome, Italy.

Marco Pierini (M)

Department of Chemistry and Technology of Drugs, Department of Excellence 2018-2022, Sapienza University of Rome, P.le Aldo Moro 5, 00185, Rome, Italy.

Bruno Botta (B)

Department of Chemistry and Technology of Drugs, Department of Excellence 2018-2022, Sapienza University of Rome, P.le Aldo Moro 5, 00185, Rome, Italy.
ORCID: 0000-0001-8707-4333

Franco Mazzei (F)

Department of Chemistry and Technology of Drugs, Department of Excellence 2018-2022, Sapienza University of Rome, P.le Aldo Moro 5, 00185, Rome, Italy.

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Globulines › Sérum-globulines › Immunoglobulines › Anticorps › Site-Directed Antibody Immobilization by...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines immobilisées › Anticorps immobilisés › Site-Directed Antibody Immobilization by...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Sites de fixation › Site-Directed Antibody Immobilization by...
questionsmedicales.fr › Produits chimiques inorganiques › Métaux › Métaux lourds › Or › Site-Directed Antibody Immobilization by...
questionsmedicales.fr › Techniques d'investigation › Techniques de sonde moléculaire › Techniques de biocapteur › Résonance plasmonique de surface › Site-Directed Antibody Immobilization by...