Metagenomics analysis reveals features unique to Indian distal gut microbiota.
Adult
Bifidobacterium
/ genetics
Body Mass Index
Carbohydrate Metabolism
/ physiology
DNA, Bacterial
/ genetics
Diet
Eating
Feces
/ microbiology
Female
Gastrointestinal Microbiome
/ genetics
Healthy Volunteers
Humans
India
Male
Metagenomics
/ methods
Phylogeny
Prevotella
/ genetics
Whole Genome Sequencing
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2020
2020
Historique:
received:
08
06
2019
accepted:
18
03
2020
entrez:
9
4
2020
pubmed:
9
4
2020
medline:
16
7
2020
Statut:
epublish
Résumé
Various factors including diet, age, geography, culture and socio-economic status have a role in determining the composition of the human gut microbiota. The human gut microbial composition is known to be altered in disease conditions. Considering the important role of the gut microbiome in maintaining homeostasis and overall health, it is important to understand the microbial diversity and the functional metagenome of the healthy gut. Here, we characterized the microbiota of 31 fecal samples from healthy individuals of Indian ethnic tribes from Ladakh, Jaisalmer and Khargone by shotgun metagenomic sequencing. Sequence analysis revealed that Bifidobacterium and Prevotella were the key microbes contributing to the differences among Jaisalmer, Khargone and Ladakh samples at the genus level. Our correlation network study identified carbohydrate-active enzymes and carbohydrate binding proteins that are associated with specific genera in the different Indian geographical regions studied. Network analysis of carbohydrate-active enzymes and genus abundance revealed that the presence of different carbohydrate-active enzymes is driven by differential abundance of genera. The correlation networks were different in the different geographical regions, and these interactions suggest the role of less abundant genera in shaping the gut environment. We compared our data with samples from different countries and found significant differences in taxonomic composition and abundance of carbohydrate-active enzymes in the gut microbiota as compared to the other countries.
Identifiants
pubmed: 32267865
doi: 10.1371/journal.pone.0231197
pii: PONE-D-19-16291
pmc: PMC7141701
doi:
Substances chimiques
DNA, Bacterial
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0231197Commentaires et corrections
Type : ErratumIn
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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