Measles virus nucleo- and phosphoproteins form liquid-like phase-separated compartments that promote nucleocapsid assembly.
Magnetic Resonance Spectroscopy
Measles
/ virology
Measles virus
/ physiology
Nucleocapsid
/ metabolism
Nucleoproteins
/ chemistry
Phosphoproteins
/ chemistry
Protein Binding
Protein Interaction Domains and Motifs
RNA, Viral
Recombinant Proteins
Thermodynamics
Viral Proteins
/ metabolism
Virus Assembly
Virus Replication
Journal
Science advances
ISSN: 2375-2548
Titre abrégé: Sci Adv
Pays: United States
ID NLM: 101653440
Informations de publication
Date de publication:
04 2020
04 2020
Historique:
received:
02
10
2019
accepted:
09
01
2020
entrez:
10
4
2020
pubmed:
10
4
2020
medline:
16
12
2020
Statut:
epublish
Résumé
Many viruses are known to form cellular compartments, also called viral factories. Paramyxoviruses, including measles virus, colocalize their proteomic and genomic material in puncta in infected cells. We demonstrate that purified nucleoproteins (N) and phosphoproteins (P) of measles virus form liquid-like membraneless organelles upon mixing in vitro. We identify weak interactions involving intrinsically disordered domains of N and P that are implicated in this process, one of which is essential for phase separation. Fluorescence allows us to follow the modulation of the dynamics of N and P upon droplet formation, while NMR is used to investigate the thermodynamics of this process. RNA colocalizes to droplets, where it triggers assembly of N protomers into nucleocapsid-like particles that encapsidate the RNA. The rate of encapsidation within droplets is enhanced compared to the dilute phase, revealing one of the roles of liquid-liquid phase separation in measles virus replication.
Identifiants
pubmed: 32270045
doi: 10.1126/sciadv.aaz7095
pii: aaz7095
pmc: PMC7112944
doi:
Substances chimiques
Nucleoproteins
0
Phosphoproteins
0
RNA, Viral
0
Recombinant Proteins
0
Viral Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
eaaz7095Informations de copyright
Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).
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