The transcription and export complex THO/TREX contributes to transcription termination in plants.
Journal
PLoS genetics
ISSN: 1553-7404
Titre abrégé: PLoS Genet
Pays: United States
ID NLM: 101239074
Informations de publication
Date de publication:
04 2020
04 2020
Historique:
received:
16
07
2019
accepted:
22
03
2020
revised:
23
04
2020
pubmed:
14
4
2020
medline:
4
8
2020
entrez:
14
4
2020
Statut:
epublish
Résumé
Transcription termination has important regulatory functions, impacting mRNA stability, localization and translation potential. Failure to appropriately terminate transcription can also lead to read-through transcription and the synthesis of antisense RNAs which can have profound impact on gene expression. The Transcription-Export (THO/TREX) protein complex plays an important role in coupling transcription with splicing and export of mRNA. However, little is known about the role of the THO/TREX complex in the control of transcription termination. In this work, we show that two proteins of the THO/TREX complex, namely TREX COMPONENT 1 (TEX1 or THO3) and HYPER RECOMBINATION1 (HPR1 or THO1) contribute to the correct transcription termination at several loci in Arabidopsis thaliana. We first demonstrate this by showing defective termination in tex1 and hpr1 mutants at the nopaline synthase (NOS) terminator present in a T-DNA inserted between exon 1 and 3 of the PHO1 locus in the pho1-7 mutant. Read-through transcription beyond the NOS terminator and splicing-out of the T-DNA resulted in the generation of a near full-length PHO1 mRNA (minus exon 2) in the tex1 pho1-7 and hpr1 pho1-7 double mutants, with enhanced production of a truncated PHO1 protein that retained phosphate export activity. Consequently, the strong reduction of shoot growth associated with the severe phosphate deficiency of the pho1-7 mutant was alleviated in the tex1 pho1-7 and hpr1 pho1-7 double mutants. Additionally, we show that RNA termination defects in tex1 and hpr1 mutants leads to 3'UTR extensions in several endogenous genes. These results demonstrate that THO/TREX complex contributes to the regulation of transcription termination.
Identifiants
pubmed: 32282821
doi: 10.1371/journal.pgen.1008732
pii: PGENETICS-D-19-01170
pmc: PMC7179932
doi:
Substances chimiques
Arabidopsis Proteins
0
EMU protein, Arabidopsis
0
PHO1 protein, Arabidopsis
0
TEX1 protein, Arabidopsis
0
Amino Acid Oxidoreductases
EC 1.4.-
nopaline synthase
EC 1.5.1.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1008732Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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