Kobuviruses carried by Rattus norvegicus in Guangdong, China.
Genetic characteristic
Kobuvirus
Prevalence
Rattus norvegicus
Journal
BMC microbiology
ISSN: 1471-2180
Titre abrégé: BMC Microbiol
Pays: England
ID NLM: 100966981
Informations de publication
Date de publication:
15 04 2020
15 04 2020
Historique:
received:
07
02
2020
accepted:
27
03
2020
entrez:
17
4
2020
pubmed:
17
4
2020
medline:
27
5
2021
Statut:
epublish
Résumé
Murine kobuviruses (MuKV) are newly recognized picornaviruses first detected in murine rodents in the USA in 2011. Little information on MuKV epidemiology in murine rodents is available. Therefore, we conducted a survey of the prevalence and genomic characteristics of rat kobuvirus in Guangdong, China. Fecal samples from 223 rats (Rattus norvegicus) were collected from Guangdong and kobuviruses were detected in 12.6% (28) of samples. Phylogenetic analysis based on partial 3D and complete VP1 sequence regions showed that rat kobuvirus obtained in this study were genetically closely related to those of rat/mouse kobuvirus reported in other geographical areas. Two near full-length rat kobuvirus genomes (MM33, GZ85) were acquired and phylogenetic analysis of these revealed that they shared very high nucleotide/amino acids identity with one another (95.4%/99.4%) and a sewage-derived sequence (86.9%/93.5% and 87.5%/93.7%, respectively). Comparison with original Aichivirus A strains, such human kobuvirus, revealed amino acid identity values of approximately 80%. Our findings indicate that rat kobuvirus have distinctive genetic characteristics from other Aichivirus A viruses. Additionally, rat kobuvirus may spread via sewage.
Sections du résumé
BACKGROUND
Murine kobuviruses (MuKV) are newly recognized picornaviruses first detected in murine rodents in the USA in 2011. Little information on MuKV epidemiology in murine rodents is available. Therefore, we conducted a survey of the prevalence and genomic characteristics of rat kobuvirus in Guangdong, China.
RESULTS
Fecal samples from 223 rats (Rattus norvegicus) were collected from Guangdong and kobuviruses were detected in 12.6% (28) of samples. Phylogenetic analysis based on partial 3D and complete VP1 sequence regions showed that rat kobuvirus obtained in this study were genetically closely related to those of rat/mouse kobuvirus reported in other geographical areas. Two near full-length rat kobuvirus genomes (MM33, GZ85) were acquired and phylogenetic analysis of these revealed that they shared very high nucleotide/amino acids identity with one another (95.4%/99.4%) and a sewage-derived sequence (86.9%/93.5% and 87.5%/93.7%, respectively). Comparison with original Aichivirus A strains, such human kobuvirus, revealed amino acid identity values of approximately 80%.
CONCLUSION
Our findings indicate that rat kobuvirus have distinctive genetic characteristics from other Aichivirus A viruses. Additionally, rat kobuvirus may spread via sewage.
Identifiants
pubmed: 32295529
doi: 10.1186/s12866-020-01767-x
pii: 10.1186/s12866-020-01767-x
pmc: PMC7161169
doi:
Substances chimiques
Capsid Proteins
0
Sewage
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
94Subventions
Organisme : This work was supported by the National Natural Science Foundation of China (grant No. 81973107).
ID : 81973107
Pays : International
Références
Vet Microbiol. 2019 Aug;235:319-326
pubmed: 31383319
Infect Genet Evol. 2019 Oct;74:103939
pubmed: 31247336
Infect Genet Evol. 2019 Nov;75:103988
pubmed: 31377399
mBio. 2018 Apr 17;9(2):
pubmed: 29666290
Rev Med Virol. 2011 Jan;21(1):32-41
pubmed: 21294214
Arch Virol. 2009;154(1):101-8
pubmed: 19096904
J Virol. 2012 Nov;86(22):12161-75
pubmed: 22933275
Infect Genet Evol. 2010 Oct;10(7):950-4
pubmed: 20547246
mBio. 2014 Oct 14;5(5):e01933-14
pubmed: 25316698
BMC Vet Res. 2019 Jul 19;15(1):254
pubmed: 31324182
Intervirology. 2017;60(5):196-200
pubmed: 29502122
Virusdisease. 2014;25(2):195-200
pubmed: 25674585
Virus Genes. 2017 Aug;53(4):593-602
pubmed: 28484931
Arch Virol. 2013 Sep;158(9):2023-30
pubmed: 23580178
Virus Evol. 2018 Feb 13;4(1):vey002
pubmed: 29449965
J Infect Dis. 1991 Nov;164(5):954-7
pubmed: 1658159
PLoS Pathog. 2011 Sep;7(9):e1002218
pubmed: 21909269
Biochem Genet. 2012 Jun;50(5-6):440-7
pubmed: 22193288