Purification of GNA-Related Lectins from Natural Sources.

Chromatography, Affinity Chromatography, Ion Exchange Galanthus / metabolism Mannose / chemistry Mannose-Binding Lectins / isolation & purification Plant Lectins / isolation & purification Plant Roots / metabolism Sepharose / chemistry
Agglutination activity GNA Glycan binding Plant lectin Snowdrop

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2020
Historique:
entrez: 20 4 2020
pubmed: 20 4 2020
medline: 9 3 2021
Statut: ppublish

Résumé

The Galanthus nivalis lectin, abbreviated as GNA, is the model protein for a large group of mannose-binding lectins. Here, we describe the purification of GNA starting from dry bulbs. Using a combination of ion exchange chromatography and affinity chromatography on mannose-Sepharose, a highly pure preparation of GNA can be obtained.

Identifiants

DOI: 10.1007/978-1-0716-0430-4_40 PMID: 32306348
pubmed: 32306348
doi: 10.1007/978-1-0716-0430-4_40
doi:

Substances chimiques

Mannose-Binding Lectins 0
Plant Lectins 0
snowdrop lectin 0
Sepharose 9012-36-6
Mannose PHA4727WTP

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

413-419

Auteurs

Els J M Van Damme (EJM)

Laboratory of Glycobiology and Biochemistry, Department of Biotechnology, Faculty of Bioscience Engineering, Ghent University, Gent, Belgium. ElsJM.VanDamme@UGent.be.

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Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Chromatographie › Chromatographie en phase liquide › Chromatographie d'affinité › Purification of GNA-Related Lectins from...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Chromatographie › Chromatographie en phase liquide › Chromatographie d'échange d'ions › Purification of GNA-Related Lectins from...
questionsmedicales.fr › Eucaryotes › Viridiplantae › Streptophyta › Embryophyta › Tracheobionta › Magnoliopsida › Lilianae › Asparagales › Amaryllidaceae › Galanthus › Purification of GNA-Related Lectins from...
questionsmedicales.fr › Glucides › Sucres › Oses › Hexose › Mannose › Purification of GNA-Related Lectins from...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Lectines › Lectines liant le mannose › Purification of GNA-Related Lectins from...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines végétales › Lectines végétales › Purification of GNA-Related Lectins from...
questionsmedicales.fr › Structures de plante › Racines de plante › Purification of GNA-Related Lectins from...
questionsmedicales.fr › Glucides › Polyosides › Agarose › Purification of GNA-Related Lectins from...