Transcriptome profiling of spike provides expression features of genes related to terpene biosynthesis in lavender.
Biosynthetic Pathways
/ genetics
Cluster Analysis
Flowers
/ genetics
Gene Expression Profiling
Gene Expression Regulation, Developmental
Gene Expression Regulation, Plant
Genes, Plant
Lavandula
/ genetics
Molecular Sequence Annotation
Oils, Volatile
/ metabolism
Phylogeny
RNA, Messenger
/ genetics
Reproducibility of Results
Terpenes
/ metabolism
Transcription Factors
/ metabolism
Transcriptome
/ genetics
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
24 04 2020
24 04 2020
Historique:
received:
12
07
2019
accepted:
07
04
2020
entrez:
26
4
2020
pubmed:
26
4
2020
medline:
1
12
2020
Statut:
epublish
Résumé
Lavender (Lavandula angustifolia) is an important economic plant because of the value of its essential oil (EO). The Yili Valley in Xinjiang has become the largest lavender planting base in China. However, there is a lack of research on the gene expression regulation of EO biosynthesis and metabolism in local varieties. Here, de novo transcriptome analysis of inflorescence of three development stages from initial flower bud to flowering stage 50% from two lavender cultivars with contrasting EO production revealed the dynamics of 100,177 differentially expressed transcripts (DETs) in various stages of spike development within and across the cultivars. The lavender transcriptome contained 77 DETs with annotations related to terpenoid biosynthesis. The expression profiles of the 27 genes involved in the methylerythritol phosphate (MEP) pathway, 22 genes in the mevalonate (MVA) pathway, 28 genes related to monoterpene and sesquiterpene biosynthesis during inflorescence development were comprehensively characterized, and possible links between the expression changes of genes and contents of EO constituents were explored. The upregulated genes were mainly concentrated in the MEP pathway, while most genes in the MVA pathway were downregulated during flower development, and cultivars with a higher EO content presented higher expression of genes in the MEP pathway, indicating that EOs were chiefly produced through the MEP pathway. Additionally, MYB transcription factors constituted the largest number of transcripts in all samples, suggesting their potential roles in regulating EO biosynthesis. The sequences and transcriptional patterns of the transcripts will be helpful for understanding the molecular basis of lavender terpene biosynthesis.
Identifiants
pubmed: 32332830
doi: 10.1038/s41598-020-63950-4
pii: 10.1038/s41598-020-63950-4
pmc: PMC7181790
doi:
Substances chimiques
Oils, Volatile
0
RNA, Messenger
0
Terpenes
0
Transcription Factors
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
6933Références
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