The BRCA2-MEILB2-BRME1 complex governs meiotic recombination and impairs the mitotic BRCA2-RAD51 function in cancer cells.
Alleles
Animals
BRCA2 Protein
/ metabolism
Cell Line, Tumor
Chromosome Pairing
DNA Breaks, Double-Stranded
Homologous Recombination
/ genetics
Male
Meiosis
/ genetics
Mice, Inbred C57BL
Mitosis
/ genetics
Multiprotein Complexes
/ metabolism
Neoplasms
/ genetics
Protein Binding
Protein Stability
Rad51 Recombinase
/ metabolism
Spermatozoa
/ metabolism
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
28 04 2020
28 04 2020
Historique:
received:
25
12
2019
accepted:
03
04
2020
entrez:
30
4
2020
pubmed:
30
4
2020
medline:
30
7
2020
Statut:
epublish
Résumé
Breast cancer susceptibility gene II (BRCA2) is central in homologous recombination (HR). In meiosis, BRCA2 binds to MEILB2 to localize to DNA double-strand breaks (DSBs). Here, we identify BRCA2 and MEILB2-associating protein 1 (BRME1), which functions as a stabilizer of MEILB2 by binding to an α-helical N-terminus of MEILB2 and preventing MEILB2 self-association. BRCA2 binds to the C-terminus of MEILB2, resulting in the formation of the BRCA2-MEILB2-BRME1 ternary complex. In Brme1 knockout (Brme1
Identifiants
pubmed: 32345962
doi: 10.1038/s41467-020-15954-x
pii: 10.1038/s41467-020-15954-x
pmc: PMC7188823
doi:
Substances chimiques
BRCA2 Protein
0
Multiprotein Complexes
0
Rad51 Recombinase
EC 2.7.7.-
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2055Subventions
Organisme : NIGMS NIH HHS
ID : R35 GM118052
Pays : United States
Organisme : Wellcome Trust
ID : 104158/Z/14/Z
Pays : United Kingdom
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