Direct Sequencing of tRNA by 2D-HELS-AA MS Seq Reveals Its Different Isoforms and Dynamic Base Modifications.

Algorithms Base Pairing Hydrophobic and Hydrophilic Interactions Isomerism Mass Spectrometry / methods RNA Processing, Post-Transcriptional RNA, Transfer / chemistry Sequence Analysis, RNA / methods

Journal

ACS chemical biology
ISSN: 1554-8937
Titre abrégé: ACS Chem Biol
Pays: United States
ID NLM: 101282906

Informations de publication

Date de publication:
19 06 2020
Historique:
pubmed: 5 5 2020
medline: 27 4 2021
entrez: 5 5 2020
Statut: ppublish

Résumé

Post-transcriptional modifications are intrinsic to RNA structure and function. However, methods to sequence RNA typically require a cDNA intermediate and are either not able to sequence these modifications or are tailored to sequence one specific nucleotide modification only. Interestingly, some of these modifications occur with <100% frequency at their particular sites, and site-specific quantification of their stoichiometries is another challenge. Here, we report a direct method for sequencing tRNA

Identifiants

DOI: 10.1021/acschembio.0c00119 PMID: 32364699 PMC: PMC7902080
pubmed: 32364699
doi: 10.1021/acschembio.0c00119
pmc: PMC7902080
mid: NIHMS1667131
doi:

Substances chimiques

RNA, Transfer 9014-25-9

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1464-1472

Subventions

Organisme : NHGRI NIH HHS
ID : R21 HG009576
Pays : United States

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Auteurs

Ning Zhang (N)

Department of Biological and Chemical Sciences, New York Institute of Technology, New York, New York 10023, United States.
Department of Chemical Engineering, Columbia University, New York, New York 10027, United States.
ORCID: 0000-0002-8103-8224

Shundi Shi (S)

Department of Chemical Engineering, Columbia University, New York, New York 10027, United States.

Xuanting Wang (X)

Department of Chemical Engineering, Columbia University, New York, New York 10027, United States.

Wenhao Ni (W)

Department of Biological and Chemical Sciences, New York Institute of Technology, New York, New York 10023, United States.

Xiaohong Yuan (X)

Department of Biological and Chemical Sciences, New York Institute of Technology, New York, New York 10023, United States.

Jiachen Duan (J)

Department of Biological and Chemical Sciences, New York Institute of Technology, New York, New York 10023, United States.

Tony Z Jia (TZ)

Earth-Life Science Institute, Tokyo Institute of Technology, Meguro-ku, Tokyo 152-8550, Japan.
Blue Marble Space Institute of Science, Seattle, Washington 98154, United States.
ORCID: 0000-0001-5175-4599

Barney Yoo (B)

Department of Chemistry, Hunter College, City University of New York, New York, New York 10065, United States.

Ashley Ziegler (A)

Department of Biological and Chemical Sciences, New York Institute of Technology, New York, New York 10023, United States.

James J Russo (JJ)

Department of Chemical Engineering, Columbia University, New York, New York 10027, United States.

Wenjia Li (W)

Department of Computer Science, New York Institute of Technology, New York, New York 10023, United States.

Shenglong Zhang (S)

Department of Biological and Chemical Sciences, New York Institute of Technology, New York, New York 10023, United States.

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Classifications MeSH

questionsmedicales.fr › Sciences de l'information › Méthodologies informatiques › Algorithmes › Direct Sequencing of tRNA by 2D-HELS-AA MS Seq...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Conformation d'acide nucléique › Appariement de bases › Direct Sequencing of tRNA by 2D-HELS-AA MS Seq...
questionsmedicales.fr › Phénomènes chimiques › Interactions hydrophobes et hydrophiles › Direct Sequencing of tRNA by 2D-HELS-AA MS Seq...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes de chimie organique › Isomérie › Direct Sequencing of tRNA by 2D-HELS-AA MS Seq...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Spectrométrie de masse › Direct Sequencing of tRNA by 2D-HELS-AA MS Seq...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Maturation post-transcriptionnelle des ARN › Direct Sequencing of tRNA by 2D-HELS-AA MS Seq...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN de transfert › Direct Sequencing of tRNA by 2D-HELS-AA MS Seq...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Analyse de séquence › Analyse de séquence d'ARN › Direct Sequencing of tRNA by 2D-HELS-AA MS Seq...