A Functional Network of Novel Barley MicroRNAs and Their Targets in Response to Drought.
barley
mRNA targets of novel barley microRNAs
microRNA
microRNA expression pattern
novel barley microRNAs
plant response to drought
Journal
Genes
ISSN: 2073-4425
Titre abrégé: Genes (Basel)
Pays: Switzerland
ID NLM: 101551097
Informations de publication
Date de publication:
29 04 2020
29 04 2020
Historique:
received:
23
03
2020
revised:
17
04
2020
accepted:
21
04
2020
entrez:
6
5
2020
pubmed:
6
5
2020
medline:
18
3
2021
Statut:
epublish
Résumé
The regulation of mRNA (messenger RNA) levels by microRNA-mediated activity is especially important in plant responses to environmental stresses. In this work, we report six novel barley microRNAs, including two processed from the same precursor that are severely downregulated under drought conditions. For all analyzed microRNAs, we found target genes that were upregulated under drought conditions and that were known to be involved in a plethora of processes from disease resistance to chromatin-protein complex formation and the regulation of transcription in mitochondria. Targets for novel barley microRNAs were confirmed through degradome data analysis and RT-qPCR using primers flanking microRNA-recognition site. Our results show a broad transcriptional response of barley to water deficiency conditions through microRNA-mediated gene regulation and facilitate further research on drought tolerance in crops.
Identifiants
pubmed: 32365647
pii: genes11050488
doi: 10.3390/genes11050488
pmc: PMC7290300
pii:
doi:
Substances chimiques
Chromatin
0
MicroRNAs
0
RNA, Messenger
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
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