Rewiring central carbon metabolism for tyrosol and salidroside production in Saccharomyces cerevisiae.
Saccharomyces cerevisiae
d-erythrose 4-phosphate
phosphoketolase
salidroside
tyrosol
Journal
Biotechnology and bioengineering
ISSN: 1097-0290
Titre abrégé: Biotechnol Bioeng
Pays: United States
ID NLM: 7502021
Informations de publication
Date de publication:
08 2020
08 2020
Historique:
received:
19
02
2020
revised:
01
05
2020
accepted:
03
05
2020
pubmed:
6
5
2020
medline:
11
8
2021
entrez:
6
5
2020
Statut:
ppublish
Résumé
Metabolic engineering of Saccharomyces cerevisiae for high-level production of aromatic chemicals has received increasing attention in recent years. Tyrosol production from glucose by S. cerevisiae is considered an environmentally sustainable and safe approach. However, the production of tyrosol and salidroside by engineered S. cerevisiae has been reported to be lower than 2 g/L to date. In this study, S. cerevisiae was engineered with a push-pull-restrain strategy to efficiently produce tyrosol and salidroside from glucose. The biosynthetic pathways of ethanol, phenylalanine, and tryptophan were restrained by disrupting PDC1, PHA2, and TRP3. Subsequently, tyrosol biosynthesis was enhanced with a metabolic pull strategy of introducing PcAAS and EcTyrA
Substances chimiques
Glucosides
0
Phenols
0
4-hydroxyphenylethanol
1AK4MU3SNX
Carbon
7440-44-0
Glucose
IY9XDZ35W2
rhodioloside
M983H6N1S9
Phenylethyl Alcohol
ML9LGA7468
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2410-2419Subventions
Organisme : National Key R&D Program of China
ID : 2018YFA090010
Pays : International
Organisme : Major Program of Shandong Province Natural Science Foundation
ID : ZR2018ZB0209
Pays : International
Organisme : Key Technologies R&D Program of Shandong Province
ID : 2018GSF121021
Pays : International
Organisme : 111 Project
ID : B16030
Pays : International
Organisme : State Key Laboratory of Microbial Technology Open Projects Fund and National Natural Science Foundation of China
ID : 31870785
Pays : International
Organisme : State Key Laboratory of Microbial Technology Open Projects Fund and National Natural Science Foundation of China
ID : 31570040
Pays : International
Commentaires et corrections
Type : ErratumIn
Informations de copyright
© 2020 Wiley Periodicals LLC.
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