Segregation of an MSH1 RNAi transgene produces heritable non-genetic memory in association with methylome reprogramming.
Adaptation, Physiological
/ genetics
Arabidopsis
/ genetics
Arabidopsis Proteins
/ genetics
DNA (Cytosine-5-)-Methyltransferases
/ genetics
DNA Methylation
Epigenesis, Genetic
Gene Expression Profiling
/ methods
Gene Ontology
Gene Regulatory Networks
Genome-Wide Association Study
/ methods
Histone Deacetylase 6
/ genetics
Inheritance Patterns
/ genetics
MutS DNA Mismatch-Binding Protein
/ genetics
Plants, Genetically Modified
Quantitative Trait, Heritable
RNA Interference
Signal Transduction
/ genetics
Transgenes
/ genetics
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
05 05 2020
05 05 2020
Historique:
received:
26
04
2019
accepted:
09
04
2020
entrez:
7
5
2020
pubmed:
7
5
2020
medline:
4
8
2020
Statut:
epublish
Résumé
MSH1 is a plant-specific protein. RNAi suppression of MSH1 results in phenotype variability for developmental and stress response pathways. Segregation of the RNAi transgene produces non-genetic msh1 'memory' with multi-generational inheritance. First-generation memory versus non-memory comparison, and six-generation inheritance studies, identifies gene-associated, heritable methylation repatterning. Genome-wide methylome analysis integrated with RNAseq and network-based enrichment studies identifies altered circadian clock networks, and phytohormone and stress response pathways that intersect with circadian control. A total of 373 differentially methylated loci comprising these networks are sufficient to discriminate memory from nonmemory full sibs. Methylation inhibitor 5-azacytidine diminishes the differences between memory and wild type for growth, gene expression and methylation patterning. The msh1 reprogramming is dependent on functional HISTONE DEACETYLASE 6 and methyltransferase MET1, and transition to memory requires the RNA-directed DNA methylation pathway. This system of phenotypic plasticity may serve as a potent model for defining accelerated plant adaptation during environmental change.
Identifiants
pubmed: 32371941
doi: 10.1038/s41467-020-16036-8
pii: 10.1038/s41467-020-16036-8
pmc: PMC7200659
doi:
Substances chimiques
Arabidopsis Proteins
0
MET1 protein, Arabidopsis
EC 2.1.1.-
DNA (Cytosine-5-)-Methyltransferases
EC 2.1.1.37
Histone Deacetylase 6
EC 3.5.1.98
MSH1 protein, Arabidopsis
EC 3.6.1.3
MutS DNA Mismatch-Binding Protein
EC 3.6.1.3
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
2214Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM134056
Pays : United States
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