Historic recombination in a durum wheat breeding panel enables high-resolution mapping of Fusarium head blight resistance quantitative trait loci.

Chromosome Mapping Disease Resistance / genetics Fusarium Genetic Association Studies Genetic Predisposition to Disease Genome-Wide Association Study Host-Pathogen Interactions / genetics Linkage Disequilibrium Plant Breeding Plant Diseases / genetics Quantitative Trait Loci Quantitative Trait, Heritable Recombination, Genetic Selection, Genetic Triticum / genetics

Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
05 05 2020
Historique:
received: 20 01 2020
accepted: 15 04 2020
entrez: 7 5 2020
pubmed: 7 5 2020
medline: 1 12 2020
Statut: epublish

Résumé

The durum wheat line DT696 is a source of moderate Fusarium head blight (FHB) resistance. Previous analysis using a bi-parental population identified two FHB resistance quantitative trait loci (QTL) on chromosome 5A: 5A1 was co-located with a plant height QTL, and 5A2 with a major maturity QTL. A Genome-Wide Association Study (GWAS) of DT696 derivative lines from 72 crosses based on multi-environment FHB resistance, plant height, and maturity phenotypic data was conducted to improve the mapping resolution and further elucidate the genetic relationship of height and maturity with FHB resistance. The Global Tetraploid Wheat Collection (GTWC) was exploited to identify durum wheat lines with DT696 allele and additional recombination events. The 5A2 QTL was confirmed in the derivatives, suggesting the expression stability of the 5A2 QTL in various genetic backgrounds. The GWAS led to an improved mapping resolution rendering the 5A2 interval 10 Mbp shorter than the bi-parental QTL mapping interval. Haplotype analysis using SNPs within the 5A2 QTL applied to the GTWC identified novel haplotypes and recombination breakpoints, which could be exploited for further improvement of the mapping resolution. This study suggested that GWAS of derivative breeding lines is a credible strategy for improving mapping resolution.

Identifiants

DOI: 10.1038/s41598-020-64399-1 PMID: 32372012 PMC: PMC7200731
pubmed: 32372012
doi: 10.1038/s41598-020-64399-1
pii: 10.1038/s41598-020-64399-1
pmc: PMC7200731
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

7567

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Auteurs

Ehsan Sari (E)

Aquatic and Crop Resource Development Centre, National Research Council, Saskatoon, SK, Canada.
Department of Plant Sciences, University of Saskatchewan, Saskatoon, SK, Canada.

Ron E Knox (RE)

Swift Current Research and Development Centre, Agriculture and Agri-Food Canada, Swift Current, SK, Canada. ron.knox@canada.ca.

Yuefeng Ruan (Y)

Swift Current Research and Development Centre, Agriculture and Agri-Food Canada, Swift Current, SK, Canada. yuefeng.ruan@canada.ca.

Maria Antonia Henriquez (MA)

Morden Research and Development Centre, Agriculture and Agri-Food Canada, Morden, MB, Canada.

Santosh Kumar (S)

Brandon Research and Development Centre, Agriculture and Agri-Food Canada, Brandon, MB, Canada.

Andrew J Burt (AJ)

Ottawa Research and Development Centre, Agriculture and Agri-Food Canada, Ottawa, ON, Canada.

Richard D Cuthbert (RD)

Swift Current Research and Development Centre, Agriculture and Agri-Food Canada, Swift Current, SK, Canada.

David J Konkin (DJ)

Aquatic and Crop Resource Development Centre, National Research Council, Saskatoon, SK, Canada.

Sean Walkowiak (S)

Department of Plant Sciences, University of Saskatchewan, Saskatoon, SK, Canada.
Canadian Grain Commission, Winnipeg, MB, Canada.

Heather L Campbell (HL)

Swift Current Research and Development Centre, Agriculture and Agri-Food Canada, Swift Current, SK, Canada.

Asheesh K Singh (AK)

Department of Agronomy, Iowa State University, Ames, Iowa, United States of America.

Jay Ross (J)

Swift Current Research and Development Centre, Agriculture and Agri-Food Canada, Swift Current, SK, Canada.

Prabhath Lokuruge (P)

Swift Current Research and Development Centre, Agriculture and Agri-Food Canada, Swift Current, SK, Canada.

Emma Hsueh (E)

Aquatic and Crop Resource Development Centre, National Research Council, Saskatoon, SK, Canada.

Kerry Boyle (K)

Aquatic and Crop Resource Development Centre, National Research Council, Saskatoon, SK, Canada.

Christine Sidebottom (C)

Aquatic and Crop Resource Development Centre, National Research Council, Saskatoon, SK, Canada.

Janet Condie (J)

Aquatic and Crop Resource Development Centre, National Research Council, Saskatoon, SK, Canada.

Shawn Yates (S)

Swift Current Research and Development Centre, Agriculture and Agri-Food Canada, Swift Current, SK, Canada.

Curtis J Pozniak (CJ)

Department of Plant Sciences, University of Saskatchewan, Saskatoon, SK, Canada.

Pierre R Fobert (PR)

Aquatic and Crop Resource Development Centre, National Research Council, Ottawa, ON, Canada.

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Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Cartographie chromosomique › Historic recombination in a durum wheat...
questionsmedicales.fr › Phénomènes physiologiques des plantes › Immunité des plantes › Résistance à la maladie › Historic recombination in a durum wheat...
questionsmedicales.fr › Eucaryotes › Champignons › Deuteromycota › Fusarium › Historic recombination in a durum wheat...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Études d'associations génétiques › Historic recombination in a durum wheat...
questionsmedicales.fr › Phénomènes génétiques › Génotype › Prédisposition génétique à une maladie › Historic recombination in a durum wheat...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Méthodes épidémiologiques › Épidémiologie moléculaire › Étude d'association pangénomique › Historic recombination in a durum wheat...
questionsmedicales.fr › Phénomènes biologiques › Interactions hôte-microbes › Interactions hôte-pathogène › Historic recombination in a durum wheat...
questionsmedicales.fr › Phénomènes génétiques › Liaison génétique › Déséquilibre de liaison › Historic recombination in a durum wheat...
questionsmedicales.fr › Technologie, industrie et agriculture › Agriculture › Production végétale › Amélioration des plantes › Historic recombination in a durum wheat...
questionsmedicales.fr › Phénomènes physiologiques des plantes › Maladies des plantes › Historic recombination in a durum wheat...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Composants de génome › Locus génétiques › Locus de caractère quantitatif › Historic recombination in a durum wheat...
questionsmedicales.fr › Phénomènes génétiques › Modes de transmission héréditaire › Caractère quantitatif héréditaire › Historic recombination in a durum wheat...
questionsmedicales.fr › Phénomènes génétiques › Recombinaison génétique › Historic recombination in a durum wheat...
questionsmedicales.fr › Phénomènes génétiques › Sélection génétique › Historic recombination in a durum wheat...
questionsmedicales.fr › Eucaryotes › Viridiplantae › Streptophyta › Embryophyta › Tracheobionta › Magnoliopsida › Poaceae › Triticum › Historic recombination in a durum wheat...