Prime genome editing in rice and wheat.

CRISPR-Cas Systems Deoxyribonuclease I / metabolism Gene Editing / methods Genome, Plant Oryza / genetics Triticum / genetics

Journal

Nature biotechnology
ISSN: 1546-1696
Titre abrégé: Nat Biotechnol
Pays: United States
ID NLM: 9604648

Informations de publication

Date de publication:
05 2020
Historique:
received: 12 12 2019
accepted: 11 02 2020
entrez: 13 5 2020
pubmed: 13 5 2020
medline: 13 8 2020
Statut: ppublish

Résumé

Prime editors, which are CRISPR-Cas9 nickase (H840A)-reverse transcriptase fusions programmed with prime editing guide RNAs (pegRNAs), can edit bases in mammalian cells without donor DNA or double-strand breaks. We adapted prime editors for use in plants through codon, promoter, and editing-condition optimization. The resulting suite of plant prime editors enable point mutations, insertions and deletions in rice and wheat protoplasts. Regenerated prime-edited rice plants were obtained at frequencies of up to 21.8%.

Identifiants

DOI: 10.1038/s41587-020-0455-x PMID: 32393904
pubmed: 32393904
doi: 10.1038/s41587-020-0455-x
pii: 10.1038/s41587-020-0455-x
doi:

Substances chimiques

Deoxyribonuclease I EC 3.1.21.1

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

582-585

Subventions

Organisme : NIAID NIH HHS
ID : U01 AI142756
Pays : United States
Organisme : NHGRI NIH HHS
ID : RM1 HG009490
Pays : United States
Organisme : NIBIB NIH HHS
ID : R01 EB022376
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM118062
Pays : United States
Organisme : Howard Hughes Medical Institute
Pays : United States

Commentaires et corrections

Type : CommentIn

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Auteurs

Qiupeng Lin (Q)

State Key Laboratory of Plant Cell and Chromosome Engineering, Center for Genome Editing, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, China.
College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, China.

Yuan Zong (Y)

State Key Laboratory of Plant Cell and Chromosome Engineering, Center for Genome Editing, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, China.

Chenxiao Xue (C)

State Key Laboratory of Plant Cell and Chromosome Engineering, Center for Genome Editing, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, China.
College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, China.

Shengxing Wang (S)

State Key Laboratory of Plant Cell and Chromosome Engineering, Center for Genome Editing, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, China.

Shuai Jin (S)

State Key Laboratory of Plant Cell and Chromosome Engineering, Center for Genome Editing, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, China.
College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, China.

Zixu Zhu (Z)

State Key Laboratory of Plant Cell and Chromosome Engineering, Center for Genome Editing, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, China.
College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, China.

Yanpeng Wang (Y)

State Key Laboratory of Plant Cell and Chromosome Engineering, Center for Genome Editing, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, China.
College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, China.

Andrew V Anzalone (AV)

Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of Harvard and MIT, Cambridge, MA, USA.
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA.
Howard Hughes Medical Institute, Harvard University, Cambridge, MA, USA.

Aditya Raguram (A)

Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of Harvard and MIT, Cambridge, MA, USA.
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA.
Howard Hughes Medical Institute, Harvard University, Cambridge, MA, USA.

Jordan L Doman (JL)

Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of Harvard and MIT, Cambridge, MA, USA.
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA.
Howard Hughes Medical Institute, Harvard University, Cambridge, MA, USA.
ORCID: 0000-0002-9887-0391

David R Liu (DR)

Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of Harvard and MIT, Cambridge, MA, USA.
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA.
Howard Hughes Medical Institute, Harvard University, Cambridge, MA, USA.
ORCID: 0000-0002-9943-7557

Caixia Gao (C)

State Key Laboratory of Plant Cell and Chromosome Engineering, Center for Genome Editing, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, China. cxgao@genetics.ac.cn.
College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, China. cxgao@genetics.ac.cn.
ORCID: 0000-0003-3169-8248

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Classifications MeSH

questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Épigenèse génétique › Extinction de l'expression des gènes › Systèmes CRISPR-Cas › Prime genome editing in rice and wheat.
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Esterases › Désoxyribonucléases › Deoxyribonuclease I › Prime genome editing in rice and wheat.
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Génie génétique › Édition de gène › Prime genome editing in rice and wheat.
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Génome végétal › Prime genome editing in rice and wheat.
questionsmedicales.fr › Eucaryotes › Viridiplantae › Streptophyta › Embryophyta › Tracheobionta › Magnoliopsida › Poaceae › Oryza › Prime genome editing in rice and wheat.
questionsmedicales.fr › Eucaryotes › Viridiplantae › Streptophyta › Embryophyta › Tracheobionta › Magnoliopsida › Poaceae › Triticum › Prime genome editing in rice and wheat.