Screening of five marine-derived fungal strains for their potential to produce oxidases with laccase activities suitable for biotechnological applications.
Dyes
Laccase
Laccase-like activity
Marine-derived fungi
Trichoderma asperellum
Journal
BMC biotechnology
ISSN: 1472-6750
Titre abrégé: BMC Biotechnol
Pays: England
ID NLM: 101088663
Informations de publication
Date de publication:
12 05 2020
12 05 2020
Historique:
received:
27
03
2020
accepted:
27
04
2020
entrez:
14
5
2020
pubmed:
14
5
2020
medline:
16
6
2021
Statut:
epublish
Résumé
Environmental pollution is one of the major problems that the world is facing today. Several approaches have been taken, from physical and chemical methods to biotechnological strategies (e.g. the use of oxidoreductases). Oxidative enzymes from microorganisms offer eco-friendly, cost-effective processes amenable to biotechnological applications, such as in industrial dye decolorization. The aim of this study was to screen marine-derived fungal strains isolated from three coastal areas in Tunisia to identify laccase-like activities, and to produce and characterize active cell-free supernatants of interest for dye decolorization. Following the screening of 20 fungal strains isolated from the harbors of Sfax and Monastir (Tunisia), five strains were identified that displayed laccase-like activities. Molecular-based taxonomic approaches identified these strains as belonging to the species Trichoderma asperellum, Stemphylium lucomagnoense and Aspergillus nidulans. Among these five isolates, one T. asperellum strain (T. asperellum 1) gave the highest level of secreted oxidative activities, and so was chosen for further studies. Optimization of the growth medium for liquid cultures was first undertaken to improve the level of laccase-like activity in culture supernatants. Finally, the culture supernatant of T. asperellum 1 decolorized different synthetic dyes belonging to diverse dye families, in the presence or absence of 1-hydroxybenzotriazole (HBT) as a mediator. The optimal growth conditions to produce laccase-like active cell-free supernatants from T. asperellum 1 were 1.8 mM CuSO
Sections du résumé
BACKGROUND
Environmental pollution is one of the major problems that the world is facing today. Several approaches have been taken, from physical and chemical methods to biotechnological strategies (e.g. the use of oxidoreductases). Oxidative enzymes from microorganisms offer eco-friendly, cost-effective processes amenable to biotechnological applications, such as in industrial dye decolorization. The aim of this study was to screen marine-derived fungal strains isolated from three coastal areas in Tunisia to identify laccase-like activities, and to produce and characterize active cell-free supernatants of interest for dye decolorization.
RESULTS
Following the screening of 20 fungal strains isolated from the harbors of Sfax and Monastir (Tunisia), five strains were identified that displayed laccase-like activities. Molecular-based taxonomic approaches identified these strains as belonging to the species Trichoderma asperellum, Stemphylium lucomagnoense and Aspergillus nidulans. Among these five isolates, one T. asperellum strain (T. asperellum 1) gave the highest level of secreted oxidative activities, and so was chosen for further studies. Optimization of the growth medium for liquid cultures was first undertaken to improve the level of laccase-like activity in culture supernatants. Finally, the culture supernatant of T. asperellum 1 decolorized different synthetic dyes belonging to diverse dye families, in the presence or absence of 1-hydroxybenzotriazole (HBT) as a mediator.
CONCLUSIONS
The optimal growth conditions to produce laccase-like active cell-free supernatants from T. asperellum 1 were 1.8 mM CuSO
Identifiants
pubmed: 32398071
doi: 10.1186/s12896-020-00617-y
pii: 10.1186/s12896-020-00617-y
pmc: PMC7218534
doi:
Substances chimiques
Coloring Agents
0
Laccase
EC 1.10.3.2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
27Références
Nat Prod Rep. 2007 Oct;24(5):1142-52
pubmed: 17898901
mBio. 2019 Mar 5;10(2):
pubmed: 30837337
PLoS One. 2013 Jun 03;8(6):e65633
pubmed: 23755261
J Hazard Mater. 2007 Sep 30;148(3):768-70
pubmed: 17706354
BMC Biochem. 2010 Aug 24;11:32
pubmed: 20735824
Appl Environ Microbiol. 2005 Apr;71(4):1775-84
pubmed: 15812000
Mycologia. 2003 Nov-Dec;95(6):1013-20
pubmed: 21149010
Iranian J Environ Health Sci Eng. 2012 Dec 15;9(1):27
pubmed: 23369690
Mol Biol Evol. 2018 Jun 1;35(6):1547-1549
pubmed: 29722887
Environ Sci Pollut Res Int. 2015 Jan;22(2):1034-42
pubmed: 25106516
Mar Biotechnol (NY). 2009 Nov-Dec;11(6):725-37
pubmed: 19283431
Microbiol Res. 2009;164(2):233-41
pubmed: 17681460
J Chromatogr A. 2013 Sep 27;1309:90-5
pubmed: 23973015
Bioorg Chem. 2018 Dec;81:319-325
pubmed: 30176571
Genome Biol. 2008 Apr 09;9(4):R70
pubmed: 18397532
Springerplus. 2014 Oct 25;3:631
pubmed: 25392801
Appl Environ Microbiol. 2000 Mar;66(3):920-4
pubmed: 10698752
Appl Environ Microbiol. 1999 May;65(5):2103-11
pubmed: 10224007
Curr Genomics. 2011 Apr;12(2):104-12
pubmed: 21966248
Persoonia. 2015 Dec;35:242-63
pubmed: 26823635
Crit Rev Biotechnol. 2006 Oct-Dec;26(4):201-21
pubmed: 17095432
Mol Biol Evol. 1997 Jul;14(7):685-95
pubmed: 9254330
Crit Rev Microbiol. 2008;34(3-4):189-206
pubmed: 19003603
Saudi J Biol Sci. 2013 Oct;20(4):373-81
pubmed: 24235874
Mar Drugs. 2010 Jun 23;8(6):1920-34
pubmed: 20631875
Mycologia. 2002 Jul-Aug;94(4):651-9
pubmed: 21156538
Nat Prod Res. 2018 Jan;32(1):105-111
pubmed: 28592143
Drug Metab Rev. 1991;23(3-4):253-309
pubmed: 1935573
Chemosphere. 2006 Aug;64(6):998-1005
pubmed: 16473395
Appl Environ Microbiol. 1997 Sep;63(9):3444-50
pubmed: 16535685
PLoS One. 2017 Jun 23;12(6):e0179957
pubmed: 28644879
Nat Prod Res. 2018 Dec;32(24):2887-2892
pubmed: 29022767
Proc Natl Acad Sci U S A. 2012 Apr 17;109(16):6241-6
pubmed: 22454494
Enzyme Res. 2011;2011:248735
pubmed: 21977312
J Basic Microbiol. 2017 Oct;57(10):839-851
pubmed: 28758291
Nucleic Acids Res. 1997 Sep 1;25(17):3389-402
pubmed: 9254694
Int J Mol Sci. 2019 Apr 15;20(8):
pubmed: 30991752
J Genet Eng Biotechnol. 2017 Jun;15(1):139-150
pubmed: 30647650
Front Microbiol. 2015 Apr 10;6:269
pubmed: 25914680
Appl Biochem Biotechnol. 2019 Feb;187(2):583-611
pubmed: 30009326
J Ind Microbiol Biotechnol. 2007 Mar;34(3):233-40
pubmed: 17171552
Ann Rev Mar Sci. 2012;4:495-522
pubmed: 22457985
Chemosphere. 2014 Jul;107:240-248
pubmed: 24418068
Nat Prod Rep. 2011 Feb;28(2):290-344
pubmed: 21229157
Mar Drugs. 2018 Aug 02;16(8):
pubmed: 30072624
Mar Drugs. 2013 Jul 23;11(7):2682-94
pubmed: 23880937
3 Biotech. 2013 Apr;3(2):115-125
pubmed: 28324566
Appl Microbiol Biotechnol. 2001 Jul;56(1-2):69-80
pubmed: 11499949
Nat Prod Res. 2019 Feb;33(3):367-373
pubmed: 29553823
Nucleic Acids Res. 1994 Nov 11;22(22):4673-80
pubmed: 7984417
Mycol Res. 2003 Apr;107(Pt 4):386-7
pubmed: 12825508
Saudi J Biol Sci. 2018 Nov;25(7):1446-1453
pubmed: 30505194
Fungal Biol. 2013 Jan;117(1):52-61
pubmed: 23332833
J Environ Sci (China). 2013 Dec 1;25(12):2410-6
pubmed: 24649671
J Photochem Photobiol B. 2018 Feb;179:139-148
pubmed: 29367149