Regulation by fungal endophyte of Rhodiola crenulata from enzyme genes to metabolites based on combination of transcriptome and metabolome.
RNA-Seq
alpine vegetation
biocontrol
metabolomics
plant-endophyte beneficial interactions
Journal
Journal of the science of food and agriculture
ISSN: 1097-0010
Titre abrégé: J Sci Food Agric
Pays: England
ID NLM: 0376334
Informations de publication
Date de publication:
Sep 2020
Sep 2020
Historique:
received:
04
03
2020
revised:
15
04
2020
accepted:
13
05
2020
pubmed:
14
5
2020
medline:
16
1
2021
entrez:
14
5
2020
Statut:
ppublish
Résumé
The contents of some its crucial metabolites tend to decrease when Rhodiola crenulata is cultured at low altitude. Interestingly, it was found that an endophyte, Phialocephala fortinii, could alleviate this problem. There were 16 151 differential genes including 14 706 up-regulated and 1445 down-regulated unigenes with significant differences (P < 0.05), and a total of 1432 metabolites exhibited statistically significant (P < 0.05) metabolic differences comprising 27 different marker metabolites which showed highly significant values of VIP > 5 and P < 0.01. Results highlight differential regulation of 20 enzymatic genes that are involved in the biosynthesis of five different marker metabolites including acetaldehyde, homocysteine, cyclopropylamine, 1-pyrrolinium and halistanol sulfate. The positive physiological effect of P. fortinii on R. crenulata encompasses differential regulation in carbohydrate metabolism, lipid metabolism and secondary metabolite synthesis. © 2020 Society of Chemical Industry.
Sections du résumé
BACKGROUND
BACKGROUND
The contents of some its crucial metabolites tend to decrease when Rhodiola crenulata is cultured at low altitude. Interestingly, it was found that an endophyte, Phialocephala fortinii, could alleviate this problem.
RESULTS
RESULTS
There were 16 151 differential genes including 14 706 up-regulated and 1445 down-regulated unigenes with significant differences (P < 0.05), and a total of 1432 metabolites exhibited statistically significant (P < 0.05) metabolic differences comprising 27 different marker metabolites which showed highly significant values of VIP > 5 and P < 0.01. Results highlight differential regulation of 20 enzymatic genes that are involved in the biosynthesis of five different marker metabolites including acetaldehyde, homocysteine, cyclopropylamine, 1-pyrrolinium and halistanol sulfate.
CONCLUSIONS
CONCLUSIONS
The positive physiological effect of P. fortinii on R. crenulata encompasses differential regulation in carbohydrate metabolism, lipid metabolism and secondary metabolite synthesis. © 2020 Society of Chemical Industry.
Substances chimiques
Cyclopropanes
0
Plant Proteins
0
Homocysteine
0LVT1QZ0BA
cyclopropylamine
8PR8XTH1X1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
4483-4494Subventions
Organisme : National Natural Science Foundation of China
ID : 31270383
Organisme : National Natural Science Foundation of China
ID : 31670328
Informations de copyright
© 2020 Society of Chemical Industry.
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