tRNA-derived fragments (tRFs) regulate post-transcriptional gene expression via AGO-dependent mechanism in IL-1β stimulated chondrocytes.

3' Untranslated Regions Argonaute Proteins Cell Line Chondrocytes / drug effects Gene Expression Regulation Humans Interleukin-1beta / pharmacology Janus Kinase 3 / genetics Osteoarthritis / genetics Primary Cell Culture RNA, Messenger / drug effects RNA, Small Untranslated / genetics RNA, Transfer / genetics RNA, Transfer, Cys / genetics
Chondrocyte JAK3 Noncoding RNA Osteoarthritis Transfer RNA tRNA-derived fragments

Journal

Osteoarthritis and cartilage
ISSN: 1522-9653
Titre abrégé: Osteoarthritis Cartilage
Pays: England
ID NLM: 9305697

Informations de publication

Date de publication:
08 2020
Historique:
received: 16 11 2019
revised: 15 04 2020
accepted: 29 04 2020
pubmed: 15 5 2020
medline: 27 7 2021
entrez: 15 5 2020
Statut: ppublish

Résumé

Recent studies have shown that tRNA-derived RNA fragments (tRFs) are novel regulators of post-transcriptional gene expression. However, the expression profiles and their role in post-transcriptional gene regulation in chondrocytes is unknown. Here, we determined tRFs expression profile and explored tRF-3003a role in post-transcriptional gene regulation in IL-1β stimulated chondrocytes. We used qPCR arrays to determine tRNAs and tRFs expression in age- and sex-matched primary human OA chondrocytes and TC28/I2 cells stimulated with IL-1β. Chondrocytes were transfected with tRNA-Cys IL-1β increased the expression levels of specific tRNAs and of tRF-3003a, a type 3 tRF produced by the cleavage of tRNA-Cys We demonstrate that under pathological conditions chondrocytes display perturbations in the expression profile of specific tRNAs and tRFs. Furthermore, a specific tRF namely tRF-3003a can post-transcriptionally regulate JAK3 expression via AGO/RISC formation in chondrocytes. Identification of this novel mechanism may be of value in the design of precision therapies for OA.

Identifiants

DOI: 10.1016/j.joca.2020.04.014 PMID: 32407895 PMC: PMC8418333
pubmed: 32407895
pii: S1063-4584(20)30993-6
doi: 10.1016/j.joca.2020.04.014
pmc: PMC8418333
mid: NIHMS1594425
pii:
doi:

Substances chimiques

3' Untranslated Regions 0
Argonaute Proteins 0
Interleukin-1beta 0
RNA, Messenger 0
RNA, Small Untranslated 0
RNA, Transfer, Cys 0
RNA, Transfer 9014-25-9
JAK3 protein, human EC 2.7.10.2
Janus Kinase 3 EC 2.7.10.2

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1102-1110

Subventions

Organisme : NIAMS NIH HHS
ID : R01 AR067056
Pays : United States
Organisme : NCCIH NIH HHS
ID : R01 AT007373
Pays : United States

Informations de copyright

Copyright © 2020 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.

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Auteurs

J A Green (JA)

Department of Anatomy and Neurobiology, Northeast Ohio Medical University, Rootstown, OH, 44272, USA.

M Y Ansari (MY)

Department of Anatomy and Neurobiology, Northeast Ohio Medical University, Rootstown, OH, 44272, USA.

H C Ball (HC)

Department of Anatomy and Neurobiology, Northeast Ohio Medical University, Rootstown, OH, 44272, USA.

T M Haqqi (TM)

Department of Anatomy and Neurobiology, Northeast Ohio Medical University, Rootstown, OH, 44272, USA. Electronic address: thaqqi@neomed.edu.

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Classifications MeSH

questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Composants de génome › Gènes › Composants de gène › Régions non traduites › Régions 3' non traduites › tRNA-derived fragments (tRFs) regulate...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Nucléoprotéines › Protéines de liaison à l'ARN › Ribonucléoprotéines › Complexe réprimant l'expression de l'ARN › Protéines Argonaute › tRNA-derived fragments (tRFs) regulate...
questionsmedicales.fr › Cellules › Cellules cultivées › Lignée cellulaire › tRNA-derived fragments (tRFs) regulate...
questionsmedicales.fr › Cellules › Cellules du tissu conjonctif › Chondrocytes › tRNA-derived fragments (tRFs) regulate...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › tRNA-derived fragments (tRFs) regulate...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › tRNA-derived fragments (tRFs) regulate...
questionsmedicales.fr › Facteurs biologiques › Protéines et peptides de signalisation intercellulaire › Cytokines › Monokines › Interleukine-1 › Interleukine-1 bêta › tRNA-derived fragments (tRFs) regulate...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Protein-tyrosine kinases › Janus kinases › Janus kinase 3 › tRNA-derived fragments (tRFs) regulate...
questionsmedicales.fr › Maladies ostéomusculaires › Arthrose › tRNA-derived fragments (tRFs) regulate...
questionsmedicales.fr › Techniques d'investigation › Techniques in vitro › Techniques de culture › Techniques de culture cellulaire › Culture de cellules primaires › tRNA-derived fragments (tRFs) regulate...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN messager › tRNA-derived fragments (tRFs) regulate...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN non traduit › Petit ARN non traduit › tRNA-derived fragments (tRFs) regulate...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN de transfert › tRNA-derived fragments (tRFs) regulate...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN de transfert › ARN de transfert spécifiques des différents acides aminés › ARN de transfert de la cystéine › tRNA-derived fragments (tRFs) regulate...