Posttranslational modification of Elongation Factor P from Staphylococcus aureus.
Staphylococcus aureus
EF-P
posttranslational modifications
ribosome
stalling
Journal
FEBS open bio
ISSN: 2211-5463
Titre abrégé: FEBS Open Bio
Pays: England
ID NLM: 101580716
Informations de publication
Date de publication:
07 2020
07 2020
Historique:
received:
24
03
2020
revised:
28
04
2020
accepted:
18
05
2020
pubmed:
22
5
2020
medline:
28
10
2021
entrez:
22
5
2020
Statut:
ppublish
Résumé
Antibiotic-resistant Staphylococcus aureus is becoming a major burden on health care systems in many countries, necessitating the identification of new targets for antibiotic development. Elongation Factor P (EF-P) is a highly conserved elongation protein factor that plays an important role in protein synthesis and bacteria virulence. EF-P undergoes unique posttranslational modifications in a stepwise manner to function correctly, but experimental information on EF-P posttranslational modifications is currently lacking for S. aureus. Here, we expressed EF-P in S. aureus to analyze its posttranslational modifications by mass spectrometry and report experimental proof of 5-aminopentanol modification of S. aureus EF-P.
Identifiants
pubmed: 32436337
doi: 10.1002/2211-5463.12901
pmc: PMC7327921
doi:
Substances chimiques
Peptide Elongation Factors
0
factor EF-P
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1342-1347Informations de copyright
© 2020 The Authors. Published by FEBS Press and John Wiley & Sons Ltd.
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