Stability of RNA sequences derived from the coronavirus genome in human cells.
Base Sequence
Betacoronavirus
/ chemistry
COVID-19
Conserved Sequence
Coronaviridae
/ genetics
Coronavirus Infections
/ virology
Genome, Viral
HeLa Cells
Humans
Nucleic Acid Conformation
Pandemics
Pneumonia, Viral
/ virology
RNA Stability
RNA, Viral
/ chemistry
Severe acute respiratory syndrome-related coronavirus
/ chemistry
SARS-CoV-2
Sequence Analysis, RNA
COVID-19
Functional sequence
RNA stability
SARS-CoV
SARS-CoV-2
Virus
Journal
Biochemical and biophysical research communications
ISSN: 1090-2104
Titre abrégé: Biochem Biophys Res Commun
Pays: United States
ID NLM: 0372516
Informations de publication
Date de publication:
05 07 2020
05 07 2020
Historique:
received:
30
04
2020
accepted:
01
05
2020
pubmed:
25
5
2020
medline:
12
6
2020
entrez:
25
5
2020
Statut:
ppublish
Résumé
Most viruses inhibit the innate immune system and/or the RNA degradation processes of host cells to construct an advantageous intracellular environment for their survival. Characteristic RNA sequences within RNA virus genomes or RNAs transcribed from DNA virus genomes contribute toward this inhibition. In this study, we developed a method called "Fate-seq" to comprehensively identify the RNA sequences derived from RNA and DNA viruses, contributing RNA stability in the cells. We examined the stabilization activity of 5,924 RNA fragments derived from 26 different viruses (16 RNA viruses and 10 DNA viruses) using next-generation sequencing of these RNAs fused 3' downstream of GFP reporter RNA. With the Fate-seq approach, we detected multiple virus-derived RNA sequences that stabilized GFP reporter RNA, including sequences derived from severe acute respiratory syndrome-related coronavirus (SARS-CoV). Comparative genomic analysis revealed that these RNA sequences and their predicted secondary structures are highly conserved between SARS-CoV and the novel coronavirus, SARS-CoV-2, which is responsible for the global outbreak of the coronavirus-associated disease that emerged in December 2019 (COVID-19). These sequences have the potential to enhance the stability of viral RNA genomes, thereby augmenting viral replication efficiency and virulence.
Identifiants
pubmed: 32446559
pii: S0006-291X(20)30912-8
doi: 10.1016/j.bbrc.2020.05.008
pmc: PMC7200376
pii:
doi:
Substances chimiques
RNA, Viral
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
993-999Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare no conflict of interest.
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