Mouse Retinal Cell Atlas: Molecular Identification of over Sixty Amacrine Cell Types.
Amacrine Cells
/ classification
Animals
Female
Glycine
/ metabolism
High-Throughput Nucleotide Sequencing
Homeodomain Proteins
/ metabolism
Male
Mice
Mice, Inbred C57BL
Neuropeptides
/ metabolism
Neurotransmitter Agents
/ metabolism
Receptors, Neurotransmitter
/ classification
Retina
/ cytology
Transcription Factor 4
/ metabolism
Transcription Factors
/ genetics
gamma-Aminobutyric Acid
/ metabolism
GABA
Meis2
RNA-seq
TCF4
glycine
neuropeptide
Journal
The Journal of neuroscience : the official journal of the Society for Neuroscience
ISSN: 1529-2401
Titre abrégé: J Neurosci
Pays: United States
ID NLM: 8102140
Informations de publication
Date de publication:
01 07 2020
01 07 2020
Historique:
received:
27
02
2020
revised:
07
05
2020
accepted:
13
05
2020
pubmed:
28
5
2020
medline:
24
11
2020
entrez:
28
5
2020
Statut:
ppublish
Résumé
Amacrine cells (ACs) are a diverse class of interneurons that modulate input from photoreceptors to retinal ganglion cells (RGCs), rendering each RGC type selectively sensitive to particular visual features, which are then relayed to the brain. While many AC types have been identified morphologically and physiologically, they have not been comprehensively classified or molecularly characterized. We used high-throughput single-cell RNA sequencing to profile >32,000 ACs from mice of both sexes and applied computational methods to identify 63 AC types. We identified molecular markers for each type and used them to characterize the morphology of multiple types. We show that they include nearly all previously known AC types as well as many that had not been described. Consistent with previous studies, most of the AC types expressed markers for the canonical inhibitory neurotransmitters GABA or glycine, but several expressed neither or both. In addition, many expressed one or more neuropeptides, and two expressed glutamatergic markers. We also explored transcriptomic relationships among AC types and identified transcription factors expressed by individual or multiple closely related types. Noteworthy among these were
Identifiants
pubmed: 32457074
pii: JNEUROSCI.0471-20.2020
doi: 10.1523/JNEUROSCI.0471-20.2020
pmc: PMC7329304
doi:
Substances chimiques
Homeodomain Proteins
0
Mrg1 protein, mouse
0
Neuropeptides
0
Neurotransmitter Agents
0
Receptors, Neurotransmitter
0
Tcf4 protein, mouse
0
Transcription Factor 4
0
Transcription Factors
0
gamma-Aminobutyric Acid
56-12-2
Glycine
TE7660XO1C
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
5177-5195Subventions
Organisme : NEI NIH HHS
ID : K99 EY029360
Pays : United States
Organisme : NEI NIH HHS
ID : R00 EY029360
Pays : United States
Informations de copyright
Copyright © 2020 the authors.
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