Insights into the FMNAT Active Site of FAD Synthase: Aromaticity is Essential for Flavin Binding and Catalysis.

Bacterial Proteins / chemistry Catalytic Domain Corynebacterium / enzymology Dinitrocresols / chemistry Hydrophobic and Hydrophilic Interactions Mutation Nucleotidyltransferases / chemistry Phenylalanine / chemistry Protein Binding Tyrosine / chemistry
aromatic residues binding eukaryotic ATP:FMN:adenylyltransferase flavin biosynthesis isoalloxazine isothermal titration calorimetry prokaryotic FAD synthase rational mutagenesis steady-state kinetics

Journal

International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791

Informations de publication

Date de publication:
25 May 2020
Historique:
received: 01 05 2020
revised: 21 05 2020
accepted: 22 05 2020
entrez: 30 5 2020
pubmed: 30 5 2020
medline: 18 2 2021
Statut: epublish

Résumé

The last step in the biosynthesis of flavin adenine dinucleotide (FAD) is considered a target for the design of antimicrobial drugs because it is carried out by two non-homologous proteins in eukaryotic and prokaryotic organisms. Monofunctional FMN: adenylyltransferases (FMNAT) in Eukarya and FMNAT modules of bifunctional FAD synthases (FADS) in Prokarya belong to different structural families with dissimilar chemistry and binding modes for the substrates. In this study, we analyzed the relevance of the hydrophobic environment of the flavin isoalloxazine in the FMNAT active site of

Identifiants

DOI: 10.3390/ijms21103738 PMID: 32466340 PMC: PMC7279473
pubmed: 32466340
pii: ijms21103738
doi: 10.3390/ijms21103738
pmc: PMC7279473
pii:
doi:

Substances chimiques

Bacterial Proteins 0
Dinitrocresols 0
4,6-dinitro-o-cresol 1604ZJR09T
Tyrosine 42HK56048U
Phenylalanine 47E5O17Y3R
Nucleotidyltransferases EC 2.7.7.-
FMN adenylyltransferase EC 2.7.7.2

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : Government of Aragon-FEDER
ID : E35_20R
Organisme : Spanish Agencia Estatal de Investigación and Fondo Europeo de Desarrollo Regional
ID : BIO2016-75183-P AEI/FEDER

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Auteurs

Ana Serrano (A)

Department of Biochemistry and Molecular and Cellular Biology, Faculty of Sciences, and Institute of Biocomputation and Physics of Complex Systems (Joint Units: BIFI-IQFR and GBsC-CSIC), University of Zaragoza, E-50009 Zaragoza, Spain.
Centro de Investigaciones Biológicas Margarita Salas, CSIC, Ramiro de Maeztu 9, E-28040 Madrid, Spain.

Sonia Arilla-Luna (S)

Department of Biochemistry and Molecular and Cellular Biology, Faculty of Sciences, and Institute of Biocomputation and Physics of Complex Systems (Joint Units: BIFI-IQFR and GBsC-CSIC), University of Zaragoza, E-50009 Zaragoza, Spain.

Milagros Medina (M)

Centro de Investigaciones Biológicas Margarita Salas, CSIC, Ramiro de Maeztu 9, E-28040 Madrid, Spain.
ORCID: 0000-0001-8743-0182

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines bactériennes › Insights into the FMNAT Active Site of FAD...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Conformation moléculaire › Conformation des protéines › Éléments structuraux des protéines › Domaine catalytique › Insights into the FMNAT Active Site of FAD...
questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Bâtonnets à Gram positif › Bâtonnets asporogènes à Gram positif › Bâtonnets asporogènes irréguliers à Gram positif › Corynebacterium › Insights into the FMNAT Active Site of FAD...
questionsmedicales.fr › Composés chimiques organiques › Composés nitrés › Dinitrocrésols › Insights into the FMNAT Active Site of FAD...
questionsmedicales.fr › Phénomènes chimiques › Interactions hydrophobes et hydrophiles › Insights into the FMNAT Active Site of FAD...
questionsmedicales.fr › Phénomènes génétiques › Variation génétique › Mutation › Insights into the FMNAT Active Site of FAD...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Transferases › Phosphotransferases › Nucleotidyltransferases › Insights into the FMNAT Active Site of FAD...
questionsmedicales.fr › Acides aminés, peptides et protéines › Acides aminés › Acides aminés essentiels › Phénylalanine › Insights into the FMNAT Active Site of FAD...
questionsmedicales.fr › Métabolisme › Liaison aux protéines › Insights into the FMNAT Active Site of FAD...
questionsmedicales.fr › Acides aminés, peptides et protéines › Acides aminés › Acides aminés cycliques › Acides aminés aromatiques › Tyrosine › Insights into the FMNAT Active Site of FAD...