Gene expression of four targets in situ of the first trimester maternal-fetoplacental interface.
Basic Helix-Loop-Helix Transcription Factors
/ metabolism
Chorionic Villi
/ metabolism
Computational Biology
Decidua
/ metabolism
Female
Follistatin-Related Proteins
/ metabolism
Gene Expression
Humans
In Situ Hybridization
Insulin-Like Growth Factor Binding Protein 1
/ metabolism
Placenta
/ metabolism
Pre-Eclampsia
/ genetics
Pregnancy
Pregnancy Complications
Pregnancy Trimester, First
/ metabolism
RNA-Seq
Semaphorins
/ metabolism
Trophoblasts
/ metabolism
EPAS1
FSTL3
Functional network
IGFBP1
SEMA3C
Journal
Tissue & cell
ISSN: 1532-3072
Titre abrégé: Tissue Cell
Pays: Scotland
ID NLM: 0214745
Informations de publication
Date de publication:
Jun 2020
Jun 2020
Historique:
received:
22
08
2019
revised:
19
10
2019
accepted:
05
11
2019
entrez:
1
6
2020
pubmed:
1
6
2020
medline:
16
12
2020
Statut:
ppublish
Résumé
EPAS1, FSTL3, IGFBP1, and SEMA3C were localized to determine whether expression is decidual, trophoblastic, or both in the human first trimester maternal-fetoplacental interface. Identified on global genome-wide microarray analysis of chorionic villus sampling tissues in preclinical preeclampsia, these targets were predicted to interact by bioinformatics pathways analysis. In situ hybridization (ISH) with mRNA of each gene was conducted in 10 cases of archived first trimester termination tissues. Randomly selected areas of cells by tissue type yielded the relative proportion of cells expressing mRNA signal in decidual and fetoplacental sites. Data were analyzed using Shapiro-Wilk and Kruskal-Wallis tests (p ≤ .05). The average gestational age was 10.2 weeks. Expression signal for each gene differed by cell type (p < .001). FSTL3 expression was 17 times higher in cells of anchoring columns than areas of decidua without ISH signal. SEMA3C was three times higher in cells of anchoring columns than in decidua. EPAS1 was 1.31 times higher in cells of anchoring columns than in areas of decidua. IGFBP1 was 20 times higher in some decidua versus cells in anchoring columns or villous trophoblast. While all targets were expressed by both maternal and fetoplacental cells, our localizations identified which compartment had relatively higher expression of each gene.
Identifiants
pubmed: 32473702
pii: S0040-8166(19)30360-X
doi: 10.1016/j.tice.2019.101313
pmc: PMC7264084
mid: NIHMS1573420
pii:
doi:
Substances chimiques
Basic Helix-Loop-Helix Transcription Factors
0
Follistatin-Related Proteins
0
Fstl3 protein, human
0
Insulin-Like Growth Factor Binding Protein 1
0
Sema3C protein, human
0
Semaphorins
0
endothelial PAS domain-containing protein 1
1B37H0967P
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
101313Subventions
Organisme : NINR NIH HHS
ID : R03 NR013961
Pays : United States
Informations de copyright
Copyright © 2019 Elsevier Ltd. All rights reserved.
Références
J Pregnancy. 2016;2016:8689436
pubmed: 27239344
Adv Chronic Kidney Dis. 2013 May;20(3):271-9
pubmed: 23928393
Science. 1997 Sep 12;277(5332):1669-72
pubmed: 9287221
Circ Cardiovasc Qual Outcomes. 2017 Feb;10(2):
pubmed: 28228456
Placenta. 2016 Apr;40:52-9
pubmed: 27016783
Placenta. 2011 Jan;32(1):33-43
pubmed: 21075446
Br J Obstet Gynaecol. 1986 Oct;93(10):1049-59
pubmed: 3790464
Nurs Outlook. 2018 May - Jun;66(3):283-292
pubmed: 29573828
J Am Heart Assoc. 2017 Aug 31;6(9):
pubmed: 28860232
Front Immunol. 2018 Aug 08;9:1661
pubmed: 30135684
Endocrinology. 1992 Sep;131(3):1230-40
pubmed: 1380437
J Biol Chem. 1998 Mar 13;273(11):6482-7
pubmed: 9497382
Obstet Gynecol. 2018 Feb;131(2):313-321
pubmed: 29324603
PLoS One. 2008 Apr 30;3(4):e2078
pubmed: 18446208
Placenta. 2010 Oct;31(10):880-5
pubmed: 20692035
Oncogene. 2012 Mar 29;31(13):1710-22
pubmed: 21892205
Mol Cell Proteomics. 2014 Feb;13(2):397-406
pubmed: 24309898
Biochem Biophys Res Commun. 1988 May 16;152(3):1289-97
pubmed: 2454104
Nature. 2018 Nov;563(7731):347-353
pubmed: 30429548
Oncogene. 2011 Mar 10;30(10):1213-28
pubmed: 21057546
Endocrinology. 2005 Jan;146(1):130-6
pubmed: 15471966
PLoS One. 2015 Feb 13;10(2):e0116508
pubmed: 25679511
J Matern Fetal Neonatal Med. 2019 Aug;32(16):2680-2687
pubmed: 29478359
Proc Natl Acad Sci U S A. 2010 Aug 17;107(33):14903-8
pubmed: 20668238
Front Immunol. 2014 Mar 28;5:125
pubmed: 24734032
Cell. 1985 May;41(1):301-12
pubmed: 2986848
Placenta. 2017 Dec;60:134-139
pubmed: 28651899
Biochem Biophys Res Commun. 2018 Nov 30;506(3):692-697
pubmed: 30454705
Stroke. 2018 Mar;49(3):524-530
pubmed: 29438078
Endocrinology. 2003 Sep;144(9):3922-33
pubmed: 12933666
Hum Reprod Update. 2006 Mar-Apr;12(2):137-44
pubmed: 16234296
Obstet Gynecol. 2013 Nov;122(5):1122-31
pubmed: 24150027
FASEB J. 2012 Dec;26(12):4925-36
pubmed: 22919069
Endocrinology. 1986 Apr;118(4):1375-8
pubmed: 2419121
Blood. 2003 Feb 1;101(3):907-14
pubmed: 12393531
Growth Horm IGF Res. 2010 Dec;20(6):422-6
pubmed: 21051252
Placenta. 2013 Mar;34(3):248-55
pubmed: 23332415
Arch Gynecol Obstet. 2018 May;297(5):1175-1179
pubmed: 29450692
Sci Adv. 2018 Oct 31;4(10):eaau4788
pubmed: 30402542
Sci Rep. 2016 Oct 21;6:35489
pubmed: 27765951
EMBO J. 1988 Aug;7(8):2417-23
pubmed: 2461294
Biol Res Nurs. 2011 Apr;13(2):134-9
pubmed: 21044967
Cancer Res. 2004 Jan 15;64(2):456-62
pubmed: 14744756
Reprod Sci. 2015 Apr;22(4):402-9
pubmed: 24700053
Mol Cell Biol. 2003 Dec;23(24):9361-74
pubmed: 14645546
J Matern Fetal Neonatal Med. 2019 Apr;32(7):1063-1068
pubmed: 29082781
J Endocrinol Invest. 2003 Jul;26(7):641-5
pubmed: 14594115
J Midwifery Womens Health. 2015 Jul-Aug;60(4):360-70
pubmed: 26255798
J Clin Invest. 2003 Jan;111(1):129-39
pubmed: 12511596
J Vis Exp. 2012 Apr 27;(62):
pubmed: 22566072
Mol Cell Proteomics. 2013 Mar;12(3):736-48
pubmed: 23275444
Methods. 2010 Dec;52(4):322-31
pubmed: 20699122
J Virol Methods. 2002 Jan;99(1-2):23-32
pubmed: 11684300
Placenta. 2008 Jan;29(1):51-7
pubmed: 17959243
J Obstet Gynecol Neonatal Nurs. 2008 Mar-Apr;37(2):146-57
pubmed: 18336438
Nitric Oxide. 2014 Nov 15;42:19-23
pubmed: 25106888
FASEB J. 2014 Feb;28(2):1010-21
pubmed: 24174424
Proc Natl Acad Sci U S A. 2004 Jan 13;101(2):458-63
pubmed: 14704274
Placenta. 2009 Jan;30(1):15-24
pubmed: 19027158