Nutrient-dependent control of RNA polymerase II elongation rate regulates specific gene expression programs by alternative polyadenylation.
Enzyme Activation
/ drug effects
Gene Expression Regulation
/ drug effects
Genes, Fungal
/ genetics
Mutation
Peptide Chain Elongation, Translational
/ drug effects
Phosphates
/ pharmacology
Polyadenylation
Promoter Regions, Genetic
/ genetics
RNA Polymerase II
/ chemistry
Saccharomyces cerevisiae
/ enzymology
Schizosaccharomyces
/ enzymology
Transcription Factors
/ genetics
NTP sensing
RNA polymerase II
alternative polyadenylation
phosphate starvation
transcription elongation rate
transcription termination
Journal
Genes & development
ISSN: 1549-5477
Titre abrégé: Genes Dev
Pays: United States
ID NLM: 8711660
Informations de publication
Date de publication:
01 07 2020
01 07 2020
Historique:
received:
28
01
2020
accepted:
06
05
2020
pubmed:
6
6
2020
medline:
11
11
2020
entrez:
6
6
2020
Statut:
ppublish
Résumé
Transcription by RNA polymerase II (RNAPII) is a dynamic process with frequent variations in the elongation rate. However, the physiological relevance of variations in RNAPII elongation kinetics has remained unclear. Here we show in yeast that a RNAPII mutant that reduces the transcription elongation rate causes widespread changes in alternative polyadenylation (APA). We unveil two mechanisms by which APA affects gene expression in the slow mutant: 3' UTR shortening and gene derepression by premature transcription termination of upstream interfering noncoding RNAs. Strikingly, the genes affected by these mechanisms are enriched for functions involved in phosphate uptake and purine synthesis, processes essential for maintenance of the intracellular nucleotide pool. As nucleotide concentration regulates transcription elongation, our findings argue that RNAPII is a sensor of nucleotide availability and that genes important for nucleotide pool maintenance have adopted regulatory mechanisms responsive to reduced rates of transcription elongation.
Identifiants
pubmed: 32499400
pii: gad.337212.120
doi: 10.1101/gad.337212.120
pmc: PMC7328516
doi:
Substances chimiques
Phosphates
0
Transcription Factors
0
RNA Polymerase II
EC 2.7.7.-
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
883-897Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM115636
Pays : United States
Informations de copyright
© 2020 Yague-Sanz et al.; Published by Cold Spring Harbor Laboratory Press.
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