Resveratrol regulates the recovery of rat sciatic nerve crush injury by promoting the autophagy of Schwann cells.
Animals
Autophagy
/ drug effects
Axons
/ drug effects
Crush Injuries
/ pathology
Male
Microtubule-Associated Proteins
/ metabolism
Motor Activity
/ drug effects
Myelin P0 Protein
/ metabolism
Myelin Sheath
/ drug effects
Nerve Crush
Nerve Fibers
/ drug effects
Nerve Regeneration
/ drug effects
Rats, Sprague-Dawley
Reactive Oxygen Species
/ metabolism
Recovery of Function
/ drug effects
Resveratrol
/ pharmacology
Schwann Cells
/ drug effects
Sciatic Nerve
/ drug effects
Peripheral nerve crush injury
Resveratrol
Schwann cell autophagy
Wallerian degeneration
Journal
Life sciences
ISSN: 1879-0631
Titre abrégé: Life Sci
Pays: Netherlands
ID NLM: 0375521
Informations de publication
Date de publication:
01 Sep 2020
01 Sep 2020
Historique:
received:
25
12
2019
revised:
30
05
2020
accepted:
08
06
2020
pubmed:
13
6
2020
medline:
1
9
2020
entrez:
13
6
2020
Statut:
ppublish
Résumé
Resveratrol has the ability to promote functional recovery after sciatic nerve crush injury (SNCI), though the mechanism through which this occurs in not fully understood. Resveratrol can promote autophagy, a key process in Wallerian degeneration; thus, we hypothesized that resveratrol could promote recovery from SNCI by promoting Schwann cell autophagy and acceleration of Wallerian degeneration. Motor function recovery was assessed by calculating Sciatic Function Indexes (SFIs) at days 7, 14, 21, 28 post SNCI. Autophagy and myelin clearance were assessed by microtubule-associated protein light chain 3B (LC3B) and myelin protein zero (MPZ) immunofluorescence and Western blot analysis on the fourth day after SNCI. The autophagy of Schwann cells following resveratrol administration was quantified by immunofluorescence in RSC96 cells. Immunofluorescence and Transmission electron microscopy (TEM) were also used in Resveratrol treated sciatic nerve four days post-SNCI to find LC3B positive areas and typical double membrane structures represent for autophagy. The SNCI+resveratrol (crush+Res) groups recovered faster than the SNCI+vehicles (crush+V) group. On day four, almost all of the myelin had regenerated in the crush+Res rats, while the crush+V group's myelin remained intact and the expression levels of LC3-II/I was the highest. On day 28 post-injury, both the control and crush+Res groups' myelin neurofibers reached peak numbers as did the thickness of the myelin sheath. Both in vitro and in vivo immunofluorescence showed that LC3B was colocalized with Schwann cells. This is the first study to observe that resveratrol can promote recovery from SCNI by accelerating the myelin clearance process by promoting autophagy of Schwann cells.
Identifiants
pubmed: 32531375
pii: S0024-3205(20)30709-8
doi: 10.1016/j.lfs.2020.117959
pii:
doi:
Substances chimiques
LC3 protein, rat
0
Microtubule-Associated Proteins
0
Myelin P0 Protein
0
Reactive Oxygen Species
0
Resveratrol
Q369O8926L
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
117959Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest There are no conflicts of interests in this article.