Stability of plasmid and viral banks supporting the cGMP manufacture of Q-Griffithsin from a TMV-based viral vector.
Microbicide
Plant-made pharmaceuticals
Q-Griffithsin
Stability
TMV
Virion
Journal
Journal of biotechnology
ISSN: 1873-4863
Titre abrégé: J Biotechnol
Pays: Netherlands
ID NLM: 8411927
Informations de publication
Date de publication:
20 Aug 2020
20 Aug 2020
Historique:
received:
21
02
2020
revised:
02
06
2020
accepted:
12
06
2020
pubmed:
20
6
2020
medline:
9
2
2021
entrez:
20
6
2020
Statut:
ppublish
Résumé
The "whole genome" TMV-based expression system, Geneware®, was used in the cGMP production of the plant-made pharmaceutical Q-Griffithsin and demonstrates stable expression for up to a two-year period. Virion and plasmid banks which contained viral cDNA and a Q-Griffithsin sequence were able to produce >200 g of Q-Griffithsin. Data assessing the quality and stability of the product banks were measured through functional assessments of visual symptomology and product expression.
Identifiants
pubmed: 32553533
pii: S0168-1656(20)30156-5
doi: 10.1016/j.jbiotec.2020.06.009
pmc: PMC7397727
mid: NIHMS1607511
pii:
doi:
Substances chimiques
Anti-Infective Agents
0
Lectins
0
Plant Lectins
0
Recombinant Proteins
0
q-griffithsin
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
74-76Subventions
Organisme : NIAID NIH HHS
ID : U19 AI113182
Pays : United States
Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest KEP, JLF, and KTH are inventors on patents and patent applications that claim Q-GRFT composition and utility. In addition, KEP, JLF, and KTH are founders and equity holders in GROW Biomedicine LLC, which is commercializing Q-GRFT. EH, JMC, JWS declare no conflicts of interest.
Références
Virology. 1999 Mar 15;255(2):312-23
pubmed: 10069957
Proc Natl Acad Sci U S A. 2009 Apr 14;106(15):6099-104
pubmed: 19332801
Viruses. 2016 Oct 24;8(10):
pubmed: 27783038
Methods Mol Biol. 2016;1385:129-37
pubmed: 26614286
Front Bioeng Biotechnol. 2018 Jul 24;6:102
pubmed: 30087892
Virology. 2007 Sep 15;366(1):98-106
pubmed: 17493658
Plant Biotechnol J. 2015 Oct;13(8):1160-8
pubmed: 26176205
Plant Physiol. 2010 Aug;153(4):1859-70
pubmed: 20498336
Plant Biotechnol J. 2010 Jun;8(5):638-54
pubmed: 20514694