Filament formation by the translation factor eIF2B regulates protein synthesis in starved cells.
Budding yeast
Protein assembly
Regulation of translation
Starvation
Stress response
Journal
Biology open
ISSN: 2046-6390
Titre abrégé: Biol Open
Pays: England
ID NLM: 101578018
Informations de publication
Date de publication:
08 07 2020
08 07 2020
Historique:
pubmed:
20
6
2020
medline:
5
10
2021
entrez:
20
6
2020
Statut:
epublish
Résumé
Cells exposed to starvation have to adjust their metabolism to conserve energy and protect themselves. Protein synthesis is one of the major energy-consuming processes and as such has to be tightly controlled. Many mechanistic details about how starved cells regulate the process of protein synthesis are still unknown. Here, we report that the essential translation initiation factor eIF2B forms filaments in starved budding yeast cells. We demonstrate that filamentation is triggered by starvation-induced acidification of the cytosol, which is caused by an influx of protons from the extracellular environment. We show that filament assembly by eIF2B is necessary for rapid and efficient downregulation of translation. Importantly, this mechanism does not require the kinase Gcn2. Furthermore, analysis of site-specific variants suggests that eIF2B assembly results in enzymatically inactive filaments that promote stress survival and fast recovery of cells from starvation. We propose that translation regulation through filament assembly is an efficient mechanism that allows yeast cells to adapt to fluctuating environments.
Identifiants
pubmed: 32554487
pii: bio.046391
doi: 10.1242/bio.046391
pmc: PMC7358136
pii:
doi:
Substances chimiques
Eukaryotic Initiation Factor-2B
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2020. Published by The Company of Biologists Ltd.
Déclaration de conflit d'intérêts
Competing interestsSimon Alberti is an advisor on the scientific advisory board of Dewpoint Therapeutics.
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