Cytosolic ascorbate peroxidase 1 modulates ascorbic acid metabolism through cooperating with nitrogen regulatory protein P-II in tea plant under nitrogen deficiency stress.
Arabidopsis
/ genetics
Arabidopsis Proteins
/ genetics
Ascorbate Peroxidases
/ genetics
Ascorbic Acid
/ metabolism
Camellia sinensis
/ enzymology
Escherichia coli
/ growth & development
Nitrogen
/ physiology
PII Nitrogen Regulatory Proteins
/ genetics
Plant Proteins
/ genetics
Promoter Regions, Genetic
Stress, Physiological
/ genetics
Arabidopsis
Ascorbate peroxidase 1
Ascorbic acid
Nitrogen deficiency
Tea plant
iTRAQ
Journal
Genomics
ISSN: 1089-8646
Titre abrégé: Genomics
Pays: United States
ID NLM: 8800135
Informations de publication
Date de publication:
09 2020
09 2020
Historique:
received:
24
04
2020
revised:
25
05
2020
accepted:
15
06
2020
pubmed:
21
6
2020
medline:
18
8
2021
entrez:
21
6
2020
Statut:
ppublish
Résumé
Nitrogen (N) element is essential nutrient, and affect metabolism of secondary metabolites in higher plants. Ascorbate peroxidase (APX) plays an important role in ascorbic acid (AsA) metabolism of tea plant. However, the roles of cytosolic ascorbate peroxidase 1 (CsAPX1) in AsA metabolism under N deficiency stress in tea plant remains unclear in detail. In this work, nitrogen regulatory protein P-II (CsGLB1) and CsAPX1 were identified by isobaric tags for relative and absolute quantitation (iTRAQ) from tea plant. The cell growth rates in transgenic Escherichia coli overexpressing CsAPX1 and CsGLB1 were higher than empty vector under N sufficiency condition. Phenotype of shoots and roots, AsA accumulation, and expression levels of AtAPX1 and AtGLB1 genes were changed in transgenic Arabidopsis hosting CsAPX1 under N deficiency stress. These findings suggested that cytosolic CsAPX1 acted a regulator in AsA accumulation through cooperating with GLB1 under N deficiency stress in tea plant.
Identifiants
pubmed: 32562829
pii: S0888-7543(20)30459-6
doi: 10.1016/j.ygeno.2020.06.025
pii:
doi:
Substances chimiques
Arabidopsis Proteins
0
PII Nitrogen Regulatory Proteins
0
Plant Proteins
0
APX1 protein, Arabidopsis
EC 1.11.1.11
Ascorbate Peroxidases
EC 1.11.1.11
Nitrogen
N762921K75
Ascorbic Acid
PQ6CK8PD0R
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3497-3503Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no competing interests.