The CRP-family transcriptional regulator DevH regulates expression of heterocyst-specific genes at the later stage of differentiation in the cyanobacterium Anabaena sp. strain PCC 7120.
Anabaena
/ metabolism
Bacterial Proteins
/ metabolism
Cell Differentiation
/ genetics
Cyanobacteria
/ metabolism
DNA-Binding Proteins
/ metabolism
Gene Expression
/ genetics
Gene Expression Regulation, Bacterial
/ genetics
Multigene Family
/ genetics
Nitrogen
/ metabolism
Nitrogen Fixation
/ physiology
Nitrogenase
/ metabolism
Operon
/ genetics
Oxygen
/ metabolism
Promoter Regions, Genetic
/ genetics
Transcription Factors
/ metabolism
Transcriptional Activation
/ genetics
Anabaena
heterocyst differentiation
microoxic intracellular environment
nitrogenase
transcriptional regulation
Journal
Molecular microbiology
ISSN: 1365-2958
Titre abrégé: Mol Microbiol
Pays: England
ID NLM: 8712028
Informations de publication
Date de publication:
10 2020
10 2020
Historique:
received:
13
04
2020
revised:
20
05
2020
accepted:
22
05
2020
pubmed:
22
6
2020
medline:
14
7
2021
entrez:
22
6
2020
Statut:
ppublish
Résumé
Heterocysts are terminally differentiated cells of filamentous cyanobacteria, which are specialized for nitrogen fixation. Because nitrogenase is easily inactivated by oxygen, the intracellular environment of heterocysts is kept microoxic. In heterocysts, the oxygen-evolving photosystem II is inactivated, a heterocyst-specific envelope with an outer polysaccharide layer and an inner glycolipid layer is formed to limit oxygen entry, and oxygen consumption is activated. Heterocyst differentiation, which is accompanied by drastic morphological and physiological changes, requires strictly controlled gene expression systems. Here, we investigated the functions of a CRP-family transcriptional regulator, DevH, in the process of heterocyst differentiation. A devH-knockdown strain, devH-kd, was created by replacing the original promoter with the gifA promoter, which is repressed during heterocyst differentiation. Although devH-kd formed morphologically distinct cells with the heterocyst envelope polysaccharide layer, it was unable to grow diazotrophically. Genes involved in construction of the microoxic environment, such as cox operons and the hgl island, were not upregulated in devH-kd. Moreover, expression of the nif gene cluster was completely abolished. Although CnfR was expressed in devH-kd, the nif gene cluster was not induced even under microoxic conditions. Thus, DevH is necessary for the establishment of a microoxic environment and induction of nitrogenase in heterocysts.
Substances chimiques
Bacterial Proteins
0
DNA-Binding Proteins
0
Transcription Factors
0
devH protein, Nostoc
0
Nitrogenase
EC 1.18.6.1
Nitrogen
N762921K75
Oxygen
S88TT14065
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
553-562Informations de copyright
© 2020 John Wiley & Sons Ltd.
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