Impact of Cysteine Residues on MHC Binding Predictions and Recognition by Tumor-Reactive T Cells.
Amino Acid Sequence
Antigen Presentation
Antigens, Neoplasm
/ immunology
CD8-Positive T-Lymphocytes
/ immunology
Cells, Cultured
Cysteine
/ metabolism
Epitopes, T-Lymphocyte
/ immunology
HLA-A2 Antigen
/ metabolism
Humans
Lymphocyte Activation
Neoplasms
/ immunology
Peptides
/ metabolism
Protein Binding
Receptors, Antigen, T-Cell, alpha-beta
/ metabolism
T-Cell Antigen Receptor Specificity
Journal
Journal of immunology (Baltimore, Md. : 1950)
ISSN: 1550-6606
Titre abrégé: J Immunol
Pays: United States
ID NLM: 2985117R
Informations de publication
Date de publication:
15 07 2020
15 07 2020
Historique:
received:
25
09
2019
accepted:
14
05
2020
pubmed:
24
6
2020
medline:
16
3
2021
entrez:
24
6
2020
Statut:
ppublish
Résumé
The availability of MHC-binding prediction tools has been useful in guiding studies aimed at identifying candidate target Ags to generate reactive T cells and to characterize viral and tumor-reactive T cells. Nevertheless, prediction algorithms appear to function poorly for epitopes containing cysteine (Cys) residues, which can oxidize and form disulfide bonds with other Cys residues under oxidizing conditions, thus potentially interfering with their ability to bind to MHC molecules. Analysis of the results of HLA-A*02:01 class I binding assays carried out in the presence and absence of the reducing agent 2-ME indicated that the predicted affinity for 25% of Cys-containing epitopes was underestimated by a factor of 3 or more. Additional analyses were undertaken to evaluate the responses of human CD8
Identifiants
pubmed: 32571843
pii: jimmunol.1901173
doi: 10.4049/jimmunol.1901173
pmc: PMC7413297
mid: NIHMS1612803
doi:
Substances chimiques
Antigens, Neoplasm
0
Epitopes, T-Lymphocyte
0
HLA-A*02:01 antigen
0
HLA-A2 Antigen
0
Peptides
0
Receptors, Antigen, T-Cell, alpha-beta
0
Cysteine
K848JZ4886
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, N.I.H., Intramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
539-549Subventions
Organisme : Intramural NIH HHS
ID : Z01 BC010984
Pays : United States
Organisme : NIAID NIH HHS
ID : R21 AI134127
Pays : United States
Informations de copyright
Copyright © 2020 by The American Association of Immunologists, Inc.
Références
Cell. 1993 Sep 10;74(5):929-37
pubmed: 8104103
J Clin Invest. 2016 Dec 1;126(12):4690-4701
pubmed: 27841757
Annu Rev Immunol. 1995;13:587-622
pubmed: 7612235
Cancer Immunol Res. 2019 Apr;7(4):534-543
pubmed: 30709841
J Exp Med. 1998 Jan 19;187(2):265-70
pubmed: 9432985
Nucleic Acids Res. 2015 Jan;43(Database issue):D405-12
pubmed: 25300482
Oncoimmunology. 2018 Aug 27;7(11):e1492508
pubmed: 30377561
Immunogenetics. 2007 Sep;59(9):745-51
pubmed: 17701407
Nature. 1991 May 23;351(6324):290-6
pubmed: 1709722
J Immunol. 1994 Dec 15;153(12):5586-92
pubmed: 7527444
J Exp Med. 2005 Apr 18;201(8):1243-55
pubmed: 15837811
Biotechniques. 1992 Dec;13(6):901-5
pubmed: 1476743
J Mol Biol. 1997 Apr 18;267(5):1258-67
pubmed: 9150410
Proc Natl Acad Sci U S A. 2011 Jul 19;108(29):E323-31
pubmed: 21670269
J Immunol. 2007 Jun 15;178(12):7890-901
pubmed: 17548627
J Exp Med. 1996 Mar 1;183(3):1131-40
pubmed: 8642255
Proc Natl Acad Sci U S A. 1995 Aug 15;92(17):7976-80
pubmed: 7644523
Curr Protoc Immunol. 2013 Feb;Chapter 18:Unit 18.3.
pubmed: 23392640
Immunity. 2019 Oct 15;51(4):766-779.e17
pubmed: 31495665
Tissue Antigens. 2001 May;57(5):405-14
pubmed: 11556965
J Proteome Res. 2017 Apr 7;16(4):1806-1816
pubmed: 28244318
J Immunol. 2005 Apr 1;174(7):4415-23
pubmed: 15778407
BMC Bioinformatics. 2014 Jul 14;15:241
pubmed: 25017736
Science. 2004 Apr 23;304(5670):587-90
pubmed: 15001714
Immunogenetics. 2000 Aug;51(10):816-28
pubmed: 10970096
J Immunol. 1998 Mar 1;160(5):2099-106
pubmed: 9498746
J Immunol. 2000 Jul 15;165(2):948-55
pubmed: 10878370
Eur J Immunol. 1996 Aug;26(8):1911-8
pubmed: 8765039
J Exp Med. 1999 Jun 7;189(11):1757-64
pubmed: 10359579
Cancer Immun. 2013 Jul 15;13:15
pubmed: 23882160
Immunogenetics. 1994;40(3):238-41
pubmed: 8039832
Bioinformatics. 2015 Jul 1;31(13):2174-81
pubmed: 25717196
Immunogenetics. 2011 Dec;63(12):789-807
pubmed: 21732180
Leukemia. 2015 Mar;29(3):647-59
pubmed: 25092142
J Immunol. 2013 Dec 15;191(12):5831-9
pubmed: 24190657
Science. 2006 Sep 8;313(5792):1444-7
pubmed: 16960008
Immunity. 2017 Feb 21;46(2):315-326
pubmed: 28228285
Curr Protoc Immunol. 2010 Feb;Chapter 18:Unit 18.17
pubmed: 20143317
Nucleic Acids Res. 2015 Jan;43(Database issue):D784-8
pubmed: 25414323
J Immunol Res. 2015;2015:763461
pubmed: 26568965
Immunogenetics. 1999 Nov;50(3-4):213-9
pubmed: 10602881
Nature. 1987 Oct 8-14;329(6139):506-12
pubmed: 3309677
J Clin Invest. 2019 Mar 1;129(3):1109-1114
pubmed: 30714987
J Immunol. 2009 Jun 15;182(12):7763-75
pubmed: 19494300
Biochem Pharmacol. 1973 Dec 1;22(23):3099-108
pubmed: 4202581
Immunome Res. 2008 Jan 25;4:2
pubmed: 18221540
J Immunol. 1999 Dec 15;163(12):6360-4
pubmed: 10586024
Genes Dev. 1993 Dec;7(12B):2556-64
pubmed: 8276238
J Immunol. 2002 Sep 15;169(6):3191-9
pubmed: 12218137
Nature. 2004 Jan 15;427(6971):252-6
pubmed: 14724640
J Immunol. 2008 May 1;180(9):6116-31
pubmed: 18424733
Chem Soc Rev. 2018 Jan 2;47(1):231-268
pubmed: 29242887